Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HeLa, 2: 4T1, 3: RAW 264.7, 4: Rat
brain
Protein loading quantity:
20 μg
Exposure time: 60 s
Predicted MW: 50 kDa
Observed MW: 50 kDa
- ￥800 - 2500
- Bioss
- bsm-60668R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-60668R |
| 英文名称 | CSNK1G2 Recombinant Rabbit mAb |
| 中文名称 | 酪蛋白激酶1γ2重组兔单抗 |
| 英文别名 | Casein kinase I isoform gamma-2; EC:2.7.11.1; CKI-gamma 2; CK1G2; KC1G2_HUMAN; |
| 产品应用 | WB=1:500-2000, ICC/IF=1:50-100, Flow-Cyt=1:50-100 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CSNK1G2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 47 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases Stem Cells > Signaling Pathways > Wnt > Cytoplasmic |
| 亚基 | Monomer (By similarity). Interacts with MTA1 (short isoform) in the cytoplasm. Binds to PER1 and triggers its proteasomal degradation. Interacts with SMAD3. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Testis. |
| 翻译后修饰 | Autophosphorylated (By similarity). |
| 相似性 | Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. Contains 1 protein kinase domain. |
| 功能 | Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enables protein serine/threonine kinase activity. Involved in peptidyl-serine phosphorylation. Located in membrane. [provided by Alliance of Genome Resources, Apr 2022] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ICC/IF} | {1:50-100} |
| {Flow-Cyt} | {1:50-100} |
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CSNK1G2 Recombinant Rabbit mAb(bsm-60668R)-50ul/100ul/25ul
￥800 - 2500
