- ￥1500 - 3280
- Proteintech
- 82534-1-RR
- 2025年10月09日
- WB, FC (Intra), ELISA
- Rabbit
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse
- 宿主：
Rabbit
- 应用范围：
WB, FC (Intra), ELISA
- 靶点：
Phospho-PERK/EIF2AK3 (Thr982)
- 抗体英文名：
Phospho-PERK/EIF2AK3 (Thr982) Recombinant monoclonal antibody
- 抗体名：
Phospho-PERK/EIF2AK3 (Thr982) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | Calyculin A treated HEK-293 cells, NIH/3T3 cells, Calyculin A treated NIH/3T3 cells |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
This antibody is equivalent to Thr980 in mice.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:11200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82534-1-RR targets Phospho-PERK/EIF2AK3 (Thr982) in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra), ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2-alpha kinase 3 |
| 别名 | p PERK/EIF2AK3, p PERK/EIF2AK3 (Thr982), p PERK/EIF2AK3 Thr982, PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3 |
| 计算分子量 | 1116 aa, 125 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | BC126354 |
| 基因名称 | PERK |
| Gene ID (NCBI) | 9451 |
| RRID | AB_3085359 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NZJ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)
This antibody is equivalent to Thr980 in mice.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IF | Int Immunopharmacol TRx0237 induces apoptosis and enhances anti-PD-1 immunotherapeutic efficacy in anaplastic thyroid CancerAuthors - Qingyang Ning |
Eur J Pharmacol Dimethyl Fumarate Improves Non-Alcoholic Fatty Liver Disease by Regulating SIRT1 Signal to Inhibit MAMs Over-EnrichmentAuthors - Rui Zhang | ||
| human | WB | Sci Rep LINC00626 drives tamoxifen resistance in breast cancer cells by interaction with UPF1Authors - Hui Yuan |
| human | WB | Free Radic Biol Med Ultrasound-Responsive Taurine Lipid Nanoparticles Attenuate Oxidative Stress and Promote Macrophage Polarization for Diabetic Wound HealingAuthors - Zucheng Luo |
| human | WB | Vet Microbiol Bovine parainfluenza virus type 3 infections induce ER stress-mediated autophagy to facilitate virus replicationAuthors - Yu Han |
| human | WB | Cell Death Dis Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomibAuthors - Yu Feng |
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acids may be precipitated by addition of monovalent cations and two to three volumes of cold 95% ethanol, followed by incubation at 0 to -70 ℃. The DNA or RNA then may be pelleted by centrifugation at 10 to 13,000 x g. for 15 minutes at 4℃. A subsequent
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12F3 B 淋巴细胞作为实验对象（该细胞系只能由 IgM 转换成 IgA），敲除 Iα 后用 Anti-CD40-IL-4-TGFβ 作为诱导物，同样利用 GRO-seq，3C-HTGTS 和 ChIP-seq 等测序技术来评估 Iα 转录在 AID 之外的 SαCSR 中的潜在作用。研究人员发现，黏着蛋白介导的环挤压促进 3’RR 激活 IgH 恒定区上游转录单位和 Sm-Sx 配对，并揭示了 CH12F3 细胞只进行 IgA 重组的机制。最后，研究人员又继续探究了 CH12F3NCΔ 细胞
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