- ￥1500 - 3280
- Proteintech
- RMX00037
- 2025年10月09日
- WB, IHC, IF/ICC, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Multi-Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA
- 靶点：
PFKM
- 抗体英文名：
Multi-rAb® PFKM Multi-Recombinant antibody
- 抗体名：
Multi-rAb® PFKM Multi-Recombinant antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, Raji cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
RMX00037 targets PFKM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Multi-Recombinant |
| 产品类型 | Antibody |
| 全称 | phosphofructokinase, muscle |
| 别名 | 6-phosphofructokinase type A, ATP-dependent 6-phosphofructokinase, muscle type, ATP-PFK, EC:2.7.1.11, PFK A |
| 观测分子量 | 75-85 kDa |
| GenBank蛋白编号 | BC021203 |
| 基因名称 | PFKM |
| Gene ID (NCBI) | 5213 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | P08237 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular masses of 85, 82 and 93 kDa.
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文献和实验.) 0.1M sodium cacodylate - 10.7g in 500mls of distilled water. 0.2M sodium cacodylate - 21.4g in 500mls of distilled water. 0.4M sodium cacodylate - 42.8g in 500mls of distilled water. EPOXY RESIN (AGAR 100 ® )
电镜样品制备方法 Specimen preparation for electron microscopy
sodium cacodylate - 42.8g in 500mls of distilled water. EPOXY RESIN (AGAR 100 ® ) (Supplier: Agar Scientific, 66A Cambridge Road, Stansted Essex, CM24 8DA, U.K.) Agar 100 resin - 24g. DDSA (E.M. grade) - 13g. MNA (E.M
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