- ￥1500 - 3280
- Proteintech
- RMX00005
- 2025年10月09日
- WB, IF/ICC, IP, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Multi-Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, IF/ICC, IP, ELISA
- 靶点：
MAEA
- 抗体英文名：
Multi-rAb® MAEA Multi-Recombinant antibody
- 抗体名：
Multi-rAb® MAEA Multi-Recombinant antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, MCF-7 cells, U2OS cells |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
RMX00005 targets MAEA in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Multi-Recombinant |
| 产品类型 | Antibody |
| 全称 | macrophage erythroblast attacher |
| 别名 | Cell proliferation-inducing gene 5 protein, E3 ubiquitin-protein transferase MAEA, EC:2.3.2.27, EMLP, EMP |
| 计算分子量 | 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC001225 |
| 基因名称 | MAEA |
| Gene ID (NCBI) | 10296 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | Q7L5Y9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).
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文献和实验.) 0.1M sodium cacodylate - 10.7g in 500mls of distilled water. 0.2M sodium cacodylate - 21.4g in 500mls of distilled water. 0.4M sodium cacodylate - 42.8g in 500mls of distilled water. EPOXY RESIN (AGAR 100 ® )
电镜样品制备方法 Specimen preparation for electron microscopy
sodium cacodylate - 42.8g in 500mls of distilled water. EPOXY RESIN (AGAR 100 ® ) (Supplier: Agar Scientific, 66A Cambridge Road, Stansted Essex, CM24 8DA, U.K.) Agar 100 resin - 24g. DDSA (E.M. grade) - 13g. MNA (E.M
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