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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
recombinant protein
- 宿主:
Rabbit
- 应用范围:
WB, IF/ICC, FC (Intra), IP, ELISA
- 靶点:
HA Tag
- 抗体英文名:
HA Tag Recombinant monoclonal antibody
- 抗体名:
HA Tag Recombinant monoclonal antibody
- 规格:
100ul/500ul/1ml
| 规格: | 100ul | 产品价格: | ¥980.0 |
|---|---|---|---|
| 规格: | 500ul | 产品价格: | ¥4000.0 |
| 规格: | 1ml | 产品价格: | ¥6000.0 |
经过测试的应用
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293T cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81290-1-RR targets HA Tag in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | HA Tag |
| 别名 | HA |
| 基因名称 | HA Tag |
| Gene ID (NCBI) | 6 |
| RRID | AB_2935602 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | HATAG |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
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文献和实验| Species | Application | Title |
|---|---|---|
| IF | Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasisAuthors - Xiao Wang | |
| WB,IF | Angew Chem Int Ed Engl Small molecule-inducible and photoactivatable cellular RNA N1-methyladenosine editingAuthors - Guoyou Xie | |
| WB,IF | Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasisAuthors - Ji Geng | |
Cell Rep Med Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacityAuthors - Xingyu Zhou | ||
| WB | Theranostics LINC00982-encoded protein PRDM16-DT regulates CHEK2 splicing to suppress colorectal cancer metastasis and chemoresistanceAuthors - Hui-Fang Hu | |
| WB,IP,CoIP | Int J Biol Sci FKBP10 Promotes the Muscle Invasion of Bladder Cancer via Lamin A DysregulationAuthors - Xupeng Zhao |
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