- ￥600 - 3000
- Proteintech
- 98136-1-RR
- 2025年10月09日
- WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR
- Rabbit
- n/a
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2 - 8°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
n/a
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR
- 靶点：
IgG Isotype Control
- 抗体英文名：
Rabbit IgG Isotype Control Recombinant Antibody
- 抗体名：
Rabbit IgG Isotype Control Recombinant Antibody
- 规格：
100ug/500ug/1mg
| 规格： | 100ug | 产品价格： | ￥600.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥2200.0 |
| 规格： | 1mg | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | |
| Positive IP detected in | |
| Positive IHC detected in | Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | |
| Positive FC (Intra) detected in | |
| Positive FC detected in | |
| Positive ChIP-qPCR detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension |
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98136-1-RR targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR applications and shows reactivity with n/a samples.
| 经测试应用 | WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR |
| 文献引用应用 | IP |
| 经测试反应性 | n/a |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | IgG Isotype Control |
| 别名 | Rabbit IgG, Rabbit IgG Control, Rabbit IgG isotype, Rabbit IgG Isotype Control |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3672282 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
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文献和实验| Species | Application | Title |
|---|---|---|
| IP | Biochim Biophys Acta Mol Basis Dis Ischemia-reperfusion injury induces ZBP1-dependent PANoptosis in endothelial cellsAuthors - Yue Cui | |
| IP | Acta Pharmacol Sin Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathwayAuthors - Hai-Qi Wu |
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
against known autoantigens. These assays are still difficult to standardize and quantify. Typically, the results are presented as antibody titers within an assay system. Most assays use recombinant or purified autoantigens that are difficult to obtain
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