- ￥1350 - 3000
- Proteintech
- 68300-1-Ig
- 2025年10月09日
- WB, IHC, IF/ICC, IF-P, ELISA
- Mouse
- human, mouse, rat, pig, rabbit, chicken
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human cTnT protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig, rabbit, chicken
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, IF-P, ELISA
- 靶点：
Cardiac Troponin T
- 抗体英文名：
Cardiac Troponin T Monoclonal antibody
- 抗体名：
Cardiac Troponin T Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | rat heart tissue, pig heart tissue, rabbit heart tissue, mouse heart tissue, chicken heart tissue |
| Positive IHC detected in | rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | H9C2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68300-1-Ig targets Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig, rabbit, chicken |
| 文献引用反应性 | human, mouse, rat, rabbit |
| 免疫原 |
CatNo: Ag7716 Product name: Recombinant human cTnT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC002653 Sequence: MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | troponin T type 2 (cardiac) |
| 别名 | TNNT2, cTnT, Troponin T, cardiac muscle, 4E2D8 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 34-40 kDa |
| GenBank蛋白编号 | BC002653 |
| 基因名称 | Cardiac Troponin T |
| Gene ID (NCBI) | 7139 |
| RRID | AB_3085051 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P45379 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.
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文献和实验| Species | Application | Title |
|---|---|---|
| rabbit | IF | Adv Mater DNA Framework-Enabled Three Dimensional Organization of Antiarrhythmic Drugs for Radiofrequency Catheter AblationAuthors - Hangwei Chen |
| rat | IF | Biochem Pharmacol Trimetazidine attenuates Ischemia/Reperfusion-Induced myocardial ferroptosis by modulating the Sirt3/Nrf2-GSH system and reducing Oxidative/Nitrative stressAuthors - Mingyue Tan |
| mouse | IF | J Inflamm Res Cardamonin Attenuates Myocardial Ischemia/Reperfusion-Induced Ferroptosis Through Promoting STAT3 SignalingAuthors - Tao Yang |
| mouse | IF | Eur J Pharmacol Omentin-1 attenuates atrial fibrillation via Src/PI3K/Akt signaling-mediated anti-fibrotic effects in cardiac fibroblastsAuthors - Tian-Lun Li |
| mouse | IF | Clin Exp Hypertens Exploration and validation of signature genes and immune associations in septic cardiomyopathyAuthors - Zhengbo Zhao |
| rat | IF | J Transl Int Med WWP2 protects against sepsis-induced cardiac injury through inhibiting cardiomyocyte ferroptosisAuthors - Zhi Li |
Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes
in myocytes now makes it possible to investigate the relative phosphorylation of a given contractile protein as it resides within the intact myofilament. Contractile proteins, including troponin I (TnI), troponin T (TnT), myosin light chain 2 (MLC
抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
的特性，又具有免疫B细胞合成和分泌特异性抗体的能力。经在HAT培养液[含有次黄嘌呤(H)、氨基喋呤(A)和胸腺嘧啶核苷(T)]中进行选择性培养，未融合的脾细胞因不能在体外长期存活而死亡，未融合的骨髓瘤细胞合成DNA的主要途径被培养液中的氨基蝶呤阻断，又因缺乏次黄嘌呤-鸟嘌呤-磷酸核糖转移酶（HGPRT），不能利用培养液中的次黄嘌呤完成DNA的合成过程而死亡。只有融合的杂交瘤细胞由于从脾细胞获得了次黄嘌呤-鸟嘌呤-磷酸核糖转移酶，因此能在HAT培养液中存活和增殖。经过克隆选择，可筛选出能产生特异性单克隆抗体
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