- ￥700 - 1750
- Proteintech
- CL555-65124
- 2025年10月09日
- FC
- Mouse
- n/a
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®555 Fluorescent Dye
- 适应物种：
n/a
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
IgG1 Isotype Control
- 抗体英文名：
CoraLite®555 Mouse IgG1 Isotype Control (MOPC-21)
- 抗体名：
CoraLite®555 Mouse IgG1 Isotype Control (MOPC-21)
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥700.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1750.0 |
经过测试的应用
| Positive FC detected in |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC |
| 经测试反应性 | n/a |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant gamma 1 (G1m marker) |
| 别名 | Ighg1, MOPC-21, Igh-4, IgG1 |
| 基因名称 | IgG1 Isotype Control |
| Gene ID (NCBI) | 16017 |
| RRID | AB_3084555 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P01868 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
allotype or sometimes isotype. Typically antibodies against human antigens and made in mice so are mouse immunoglobulins usually mouse IgG1. The typical second step reagent would be goat anti-mouse IgG conjugated to FITC. However, care should be taken
Immunohistochemisty-Fluorescence Protocol
and Secondary Reagents Biotinylated donkey anti-goat IgG (Jackson Immuno Research Lab Catalog # 705-066-147) Biotinylated goat anti-mouse IgG1 (Caltag Lab Catalog # M32115) FITC-labeled goat anti-mouse IgG1 (Caltag Lab Catalog # M32001)
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