- ￥1500 - 3280
- Proteintech
- 68136-1-Ig
- 2025年10月09日
- WB, ELISA
- Mouse
- human, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human, rat
- 宿主：
Mouse
- 应用范围：
WB, ELISA
- 靶点：
Phospho-Caspase 9 (Thr125)
- 抗体英文名：
Phospho-Caspase 9 (Thr125) Monoclonal antibody
- 抗体名：
Phospho-Caspase 9 (Thr125) Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, Calyculin A treated Jurkat cells, Calyculin A treated HSC-T6 cells, Calyculin A treated HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68136-1-Ig targets Phospho-Caspase 9 (Thr125) in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | human, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | caspase 9, apoptosis-related cysteine peptidase |
| 别名 | CASP9, Caspase 9, Caspase 9/p35/p10, 1B5E11, APAF 3 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC002452 |
| 基因名称 | Caspase 9 |
| Gene ID (NCBI) | 842 |
| RRID | AB_2923663 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P55211 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46 kDa precursor protein which can be cleaved into a 35 kDa subunit and a 11 kDa subunit. Control of all caspases is tightly regulated by a series of phosphorylation events enacted by several different kinases. Caspase-9 is the most heavily phosphorylated of all caspases, with phosphorylation of at least 11 distinct residues in all three caspase-9 domains by nine kinases. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507, PMID: 29066624)
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文献和实验的PBS，4℃ 1-2小时封闭。 c. 用BSA-PBS洗涤3次，每次3分钟。 d. 加待检样品，每孔100 ul，设立阴性孔、阳性孔和空白孔；37℃1-2小时，同法洗涤。 e. 每孔加125I标记的抗鼠Ig抗体工作液100ul，37℃ 1-2小时，同法洗涤。 f. 用γ-射线闪烁计数仪分别测定各孔放射性。通常要求样品孔和阳性对照孔的放射性脉冲分别超过本底5倍和10倍。若以空白孔的放射性为基准，凡样品孔与阴性孔放射性之比大于3的样品定为阳性
CD34 分子 CD34 常用单克隆抗体或代号： MY10,ICH3 主要表达细胞： BM,En [M] 分子质量(kDa)和结构： gp115(与Ig- SFC2组一定相似性) 功 能： 调控早期造血,为CD62L的配体,外周淋巴结地址素,外周淋巴结粘附 CD34 Cell-cell adhesion molecule and cell surface glycoprotein
细胞浸润在这个乳腺髓样癌中表现为寡克隆（或多克隆）而不是单克隆增殖，提示多克隆Ig的表达对于肿瘤免疫有较好的正性作用[61]。单克隆抗体SK-1可以识别大肠癌表面的一种糖蛋白，Koda K等人给大肠癌病人静脉注射SK-1，8周后发现病人原来升高的CEA水平明显下降，据此他们推测SK-1具有抑制大肠癌细胞生长的作用[62]；Ghosh 等在带有Ehrlich ascites肿瘤的荷瘤小鼠血浆中发现并分离到三种分子量大小的IgA分子，其中的高分子量IgA（≥669kD）在体外实验中可明显增强外周血淋巴细胞及巨噬
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