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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at 2 - 8°C. Stable for one year after shipment.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
FC (Intra)
- 靶点:
Ki-67
- 抗体英文名:
Anti-Human Ki-67 Rabbit Recombinant Antibody
- 抗体名:
Anti-Human Ki-67 Rabbit Recombinant Antibody
- 规格:
100ug/500ug/1mg
| 规格: | 100ug | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥2200.0 |
| 规格: | 1mg | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive FC (Intra) detected in | PHA treated human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98143-2-RR targets Ki-67 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | antigen identified by monoclonal antibody Ki-67 |
| 别名 | MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67 |
| 计算分子量 | 359 kDa |
| GenBank蛋白编号 | NM_002417 |
| 基因名称 | KI67 |
| Gene ID (NCBI) | 4288 |
| RRID | AB_3672289 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P46013 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.
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文献和实验-67 antigen. ref: 1. Gerdes J, Schwab U, Lemke H, Stein H. 1983. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 31:13–20. 2. The Ki-67 Protein
Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67
The rapid evolution of monoclonal antibody (MAb) technology has resulted in an ever-growing repertoire of proteins becoming accessible for study using immunohistochemical techniques. Nuclear antigens represent a class of proteins
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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