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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2b, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Cardinal Red™ Fluorescent Dye
- 适应物种:
n/a
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
IgG2b Isotype Control
- 抗体英文名:
Cardinal Red™ Mouse IgG2b Isotype Control (MPC-11)
- 抗体名:
Cardinal Red™ Mouse IgG2b Isotype Control (MPC-11)
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥700.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1750.0 |
经过测试的应用
| Positive FC detected in |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65128 targets IgG2b Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC |
| 经测试反应性 | n/a |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy chain 3 (serum IgG2b) |
| 别名 | Ighg2b, MPC-11, Igh-3, IgG2b, gamma2b |
| 基因名称 | IgG2b Isotype Control |
| Gene ID (NCBI) | 16016 |
| RRID | AB_3084954 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P01867 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
the entire mouse or human luteinizing hormone/chorionic gonadotropin receptor genes (Lhcgr ) were purchased from Research Genetics (clones RP23-18D7 and RP11-73L19, respectively). Individual BAC clones were propagated in E. coli in Luria�Bertani (LB) media
D Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A D8 IgG2b, 09821D Eryth: Ter-119 IgG2b, 09081A Sca-1 PE-IgG2a (E31-161.7) 01835B PE-IgG2a Isotype control, 11025
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