- ￥5800
- Proteintech
- 84436-5-PBS
- 2025年10月09日
- WB, IHC, IF-P, Indirect ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, Indirect ELISA
- 靶点：
MPO
- 抗体英文名：
Mouse MPO Recombinant monoclonal antibody, PBS Only
- 抗体名：
Mouse MPO Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
84436-5-PBS targets MPO in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, Indirect ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | myeloperoxidase |
| 别名 | Myeloperoxidase light chain, Myeloperoxidase heavy chain, Myeloperoxidase, EC:1.11.2.2, 241839C11 |
| 计算分子量 | 81KD |
| 观测分子量 | 43 kDa, 59 kDa, 90 kDa |
| GenBank蛋白编号 | AAR99349 |
| 基因名称 | Mpo |
| Gene ID (NCBI) | 17523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P11247 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.
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文献和实验液，加入6ml DMEM完全培养基，37℃孵育过夜，再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变（CPE）出现（如用pAdTrack-CMV质粒，由于含GFP,可观察到绿色荧光）。四、重组病毒鉴定 1、转导10-14d后，收集细胞沉淀，加入2ml灭菌的PBS混悬，冻融细胞，离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清，感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d，当1/3-1/2细胞漂浮时
-DNA液，加入6ml DMEM完全培养基，37℃孵育过夜，再换液。 7 培养过程中观察细胞生长情况。约2周后可观察到细胞病变（CPE）出现（如用pAdTrack-CMV质粒，由于含GFP，可观察到绿色荧光）。 四 重组病毒鉴定 1 转导10-14d后，收集细胞沉淀，加入2ml灭菌的PBS混悬，冻融细胞，离心后收集上清保存于-80℃。 2 取30-50% 步骤1中上清，感染50-70%饱和度的25cm2 方瓶中293细胞。2-3d后出现明显细胞
/ml denatured rat tail collagen (Vitrogen; Collagen Corporation). To prepare collagen-coated dishes: Dilute Vitrogen stock (~3 mg/ml) 1:100 in sterile PBS, then completely cover the surface of a 10cm dish with 3-5 ml. Incubate at 33
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