- ￥5800
- Proteintech
- 84436-4-PBS
- 2025年10月09日
- IF-P, Sandwich ELISA, Indirect ELISA, Sample test
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
IF-P, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点：
MPO
- 抗体英文名：
Mouse MPO Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名：
Mouse MPO Recombinant monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
84436-4-PBS targets MPO as part of a matched antibody pair:
MP01284-4: 84436-3-PBS capture and 84436-4-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | IF-P, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg0702 Product name: Recombinant Mouse Myeloperoxidase protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-10*His Domain: 16-718 aa of AAR99349 Sequence: MLQTSNGATPALLGEVENSVVLSCMEEAKQLVDRAYKERRESIKRSLQSGSASPTELLFYFKQPVAGTRTAVRAADYLHVALDLLKRKLQPLWPRPFNVTDVLTPAQLNLLSVSSGCAYQDVGVTCPPNDKYRTITGHCNNRRSPTLGASNRAFVRWLPAEYEDGVSMPFGWTPGVNRNGFKVPLARQVSNAIVRFPNDQLTKDQERALMFMQWGQFLDHDITLTPEPATRFSFFTGLNCETSCLQQPPCFPLKIPPNDPRIKNQKDCIPFFRSCPACTRNNITIRNQINALTSFVDASGVYGSEDPLARKLRNLTNQLGLLAVNTRFQDNGRALMPFDSLHDDPCLLTNRSARIPCFLAGDMRSSEMPELTSMHTLFVREHNRLATQLKRLNPRWNGEKLYQEARKIVGAMVQIITYRDYLPLVLGPAAMKKYLPQYRSYNDSVDPRIANVFTNAFRYGHTLIQPFMFRLNNQYRPTGPNPRVPLSKVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIVVDEIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPSTVGELGTVLKNLELARKLMAQYGTPNNIDIWMGGVSEPLEPNGRVGQLLACLIGTQFRKLRDGDRFWWENPGVFSKQQRQALASISLPRIICDNTGITTVSKNNIFMSNTYPRDFVSCNTLPKLNLTSWKET |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | myeloperoxidase |
| 别名 | 241839F3, EC:1.11.2.2, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain |
| 计算分子量 | 81KD |
| GenBank蛋白编号 | AAR99349 |
| 基因名称 | Mpo |
| Gene ID (NCBI) | 17523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11247 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.
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文献和实验1. 抗原及酶标记抗体 ①抗原：兔抗人IgG(Rabbit Aanti-human IgG，Code No.A0423); ②酶标抗抗体：兔抗鼠IgG-HRP(Rabbit Anti-mouse IgG-HRP，Code No.P0260);均为 DAKO 公司产品，使用时按照说明书要求稀释。 4. 洗涤液(含0.05%Tween 20的PBS)：1LPBS 中加入Tween-20 500μL。 5. 封闭液(含1%牛血
蛋白质，加酶标抗抗体，保温后洗涤，加底物保温30分钟后，加酸或碱终止酶促反应，用目测或光电比色测定抗体含量。三、仪器、原料和试剂仪器聚苯乙烯96孔酶标板、微量移液管、酶联免疫阅读仪、水浴锅。原料单克隆抗体试剂1、 抗原及酶标记抗体①抗原：兔抗人IgG(Rabbit Aanti-human IgG，Code No．A0423)；②酶标抗抗体：兔抗鼠IgG-HRP(Rabbit Anti-mouse IgG-HRP，Code No．P0260)；均为DAKO 公司产品，使用时按照说明书要求稀释。4、 洗涤
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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