- ￥5800
- Proteintech
- 84416-5-PBS
- 2025年10月09日
- WB, IHC, IF-P, Indirect ELISA
- Rabbit
- mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, Indirect ELISA
- 靶点：
NKp46/NCR1
- 抗体英文名：
Rat NKp46/NCR1 Recombinant monoclonal antibody, PBS Only
- 抗体名：
Rat NKp46/NCR1 Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
84416-5-PBS targets NKp46/NCR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, Indirect ELISA |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg1469 Product name: Recombinant Rat NKp46/NCR1 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 17-258 aa of NM_057199.1 Sequence: QRINTEKQTLPKPIIWAKPSIMVTKGNSVNIWCQGAQSASEYQLYFEGSFFALERPKSSRSMNKVKFFISQMTSHTAGIYTCFYQSGELWSESSNPLKLVVTGLYDTPTLWVHPGPEVTLGENVTFSCHLKTATSKFFLLKERESNHIQHKYGNIQAEFPMGPVTRAHRGTYRCFGSYNDYAWSFPSEPVTLLITGEVENTSLAPTDPVSSLDYWEFDLSTKESGLQKDSAFWDHTAQNLIR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | natural cytotoxicity triggering receptor 1 |
| 别名 | 241811H7, Activating receptor 1, Ar1, CD335, Kilr1 |
| 计算分子量 | 37kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | NM_057199.1 |
| 基因名称 | NKp46/NCR1 |
| Gene ID (NCBI) | 117547 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9Z0H5 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).
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文献和实验液，加入6ml DMEM完全培养基，37℃孵育过夜，再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变（CPE）出现（如用pAdTrack-CMV质粒，由于含GFP,可观察到绿色荧光）。四、重组病毒鉴定 1、转导10-14d后，收集细胞沉淀，加入2ml灭菌的PBS混悬，冻融细胞，离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清，感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d，当1/3-1/2细胞漂浮时
Fusion of Mouse, Rat, or Hamster Cell
sterile PBS. Take an aliquot for a cell and viability count. Fusion Procedure. Mix the spleen and myeloma cells together in a 50 ml tube at a ratio of 2:1-5
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays. Solutions and Reagents • Freshly removed or flash frozen rat liver • PBS •Homogenization Buffer •Homogenization Buffer containing 0.5 mM mM
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