- ￥5800
- Proteintech
- 82828-15-PBS
- 2025年10月09日
- WB, Indirect ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Rabbit
- 应用范围：
WB, Indirect ELISA
- 靶点：
Phospho-Histone H3 (Ser28)
- 抗体英文名：
Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody, PBS Only
- 抗体名：
Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
82828-15-PBS targets Phospho-Histone H3 (Ser28) in WB, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | Histone H3 (Ser28), p Histone H3, p Histone H3 (Ser28), p Histone H3 Ser28, p-Histone H3 |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Histone modifications and variants have key roles in the activation and silencing of genes. Phosphorylation of histone H3 at serine 10 and serine 28 is involved in transcriptional activation of genes responding to stress or mitogen-stimulated signaling pathways. Histone H3 phosphorylated at serine 28 was located at the promoter region of the transcriptionally active, but not competent, histone H5 and β-globin genes (PMID: 17913747).
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文献和实验upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
液，加入6ml DMEM完全培养基，37℃孵育过夜，再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变（CPE）出现（如用pAdTrack-CMV质粒，由于含GFP,可观察到绿色荧光）。四、重组病毒鉴定 1、转导10-14d后，收集细胞沉淀，加入2ml灭菌的PBS混悬，冻融细胞，离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清，感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d，当1/3-1/2细胞漂浮时
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
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