- ￥5800
- Proteintech
- 82828-10-PBS
- 2025年10月09日
- WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR
- 靶点：
Phospho-Histone H3 (Ser10)
- 抗体英文名：
Phospho-Histone H3 (Ser10) Recombinant monoclonal antibody, PBS Only
- 抗体名：
Phospho-Histone H3 (Ser10) Recombinant monoclonal antibody, PBS Only
- 规格：
100ug
产品信息
82828-10-PBS targets Phospho-Histone H3 (Ser10) in WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | H3 P-S10, H3S10, H3S10Ph, Histone H3 (Ser10), p Histone H3 |
| 计算分子量 | 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | NM_003529 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.
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文献和实验/well, 6 well plate) or 4×106/10cm plate were transfected with 5' biotin linked EF52 sense, antisense or sense/antisense RNAs (100nM). Twenty-four hours later the cultures were isolated, washed 1 time in 1x PBS, genomic DNA isolated (Qiagen™ Dneasy), and exposed
实验步骤 1. Harvest cells and wash twice with ice-cold PBS. PBS and subsequent buffers can be supplemented with 5 mM sodium butyrate to retain levels of histone acetylation. 2. Resuspend cells in Triton Extraction Buffer
量。 杂交瘤细胞融合后，要进行筛选后才能使用。杂交瘤细胞分为两次：一、筛选出杂交瘤细胞;二、在初选的杂交瘤细胞中筛选出能产生特异性抗体的杂家瘤细胞，这两次筛选的方法和原理各不相同。3. 腹水制备及单抗纯化 腹水的制备过程比较简单： 第一步，提前一周用弗氏不完全佐剂0.5ml注射小鼠腹腔； 第二步，注射佐剂后一周将生长旺盛的杂交瘤细胞离心弃去培养基，用PBS或者不完全培养基重悬后注射小鼠腹腔，一般7-10天左右就可以看到小鼠腹腔明显肿大，此时可以处死小鼠采集腹水
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