GIBCO C12440500BT 国产IMDM培养基

• 基本信息：
  • 货号：C12440500BT。
  • 规格：500ml。
  • 品牌：Gibco。
  • 运输条件：常温。
  • 储存条件：2-8℃，避光保存。
  • 保质期：自生产之日起 12 个月。
• 成分特点：
  • 含有成分：L - 谷氨酰胺、酚红、丙tong酸钠、HEPES。
  • 不含成分：α- 硫代甘油、2 - 巯基乙醇。IMDM 培养基不含蛋白质、脂质或生长因子，通常需要添加 10% 胎牛血清（FBS）进行细胞培养。
• 产品优势：
  • 质量可靠：符合 cGMP 生产要求，生产设施通过 ISO 13485 标准认证，确保产品质量的稳定性和一致性。
• 适用细胞：
  • 非常适用于快速增殖的高密度细胞培养，包括 Jurkat、COS-7 和巨噬细胞等。
• 缓冲系统：
  • 使用碳酸氢钠缓冲系统（3.024g/L），需要 5-10% CO₂的环境来维持生理 pH 值。

Key Components

• With: High glucose (4.5 g/L), L-glutamine, 25 mM HEPES, sodium pyruvate (110 mg/L), phenol red, sodium bicarbonate, selenium, additional amino acids and vitamins
• Without: α-thioglycerol, 2-mercaptoethanol, iron (replaced with potassium nitrate)
• Glucose concentration：4500 mg/L (4.5 g/L)
• Buffering system：Sodium bicarbonate (3.024 g/L) + 25 mM HEPES; suitable for 5%–10% CO₂ incubator
• Osmolality：320–360 mOsm/kg
• pH：7.2–7.4 (equilibrated with 5% CO₂)
• Sterility：0.22 μm filter-sterilized; endotoxin ≤0.2 EU/mL; mycoplasma/bacteria/fungus-free

Features

• Highly nutritious formulation: A modified version of DMEM, containing selenium, enriched amino acids and vitamins; ideal for rapidly proliferating, high-density cell cultures.
• Dual buffering system: Combined sodium bicarbonate and HEPES, providing stable pH control and reducing CO₂ dependency.
• Iron-free formulation: Uses potassium nitrate instead of ferric nitrate, minimizing oxidative stress for sensitive cells.
• No reducing agents: Free of α-thioglycerol and 2-mercaptoethanol, suitable for redox-sensitive experiments.
• Animal-origin-free: No animal-derived components, low immunogenicity and high safety.
• cGMP manufacturing: ISO 13485 certified, excellent lot-to-lot consistency.

Recommended Cells & Applications

• Suspension cell lines：Jurkat, HL-60, K562.
• Adherent cell lines：COS-7, HeLa, HEK293, A549.
• Primary cells：Macrophages, lymphocytes, bone marrow cells, hematopoietic stem cells.
• Applications：High-density cell culture, protein expression, viral vector production, immune cell culture, stem cell research, drug screening.

Quality & Storage

• Shelf life：12 months (unopened, 2–8°C).
• Storage：2–8°C, protected from light; do not freeze；ship at ambient temperature.
• Intended use：For research only. Not for human/animal administration or clinical diagnostics.