Biosharp白鲨 BL1670A Neuronal 培养

产品简介
BL1670A 是一款无血清、低渗透压、成分优化的神经元基础培养基，专为产前 / 胚胎期原代神经元的长期培养、成熟与存活设计，适用于海马、皮层、纹状体等多种中枢神经元；配合 B-27 添加剂即可稳定培养，无需星形胶质细胞滋养层。
产品参数
形态：红色澄清液体，即用型基础培养基
适用细胞：胚胎 / 产前大鼠、小鼠原代神经元（海马、皮层、纹状体、黑质等）
必需添加物：推荐 B-27（50×），亦可使用 N-2
不含：血清、L - 谷氨酰胺、L - 谷氨酸、L - 天冬氨酸
培养体系：37℃、5% CO₂、饱和湿度
保存条件：2–8℃避光
有效期：12 个月
核心特点
无血清、低杂细胞：不含血清，减少批次差异与污染，神经元纯度高、状态均一。
长期维持与成熟：支持2–4 周以上原代神经元存活、突起生长与突触成熟。
配方优化、低毒性：渗透压与营养配比接近体内微环境，降低细胞应激与凋亡。
兼容性强：与 B-27、N-2 添加剂高度适配，可直接用于药物筛选、神经毒性、电生理等实验
使用要点（简化）
基础培养基：BL1670A（500 mL）
完全培养基：BL1670A + 2% B-27（50×）+ 0.5 mM L - 谷氨酰胺
接种初期：可添加 25 μM L - 谷氨酸 促进存活
抗生素：建议减半浓度使用（B-27 无血清体系解毒能力弱）
注意事项
仅供科研使用，不可用于临床诊断、治疗或人体给药
无菌操作，避免微生物污染。
避免反复冻融；开封后建议 1 个月内用完。
English Product Introduction
Product Name：Neuronal Medium (Neuronal Cell Culture Medium)
Cat. No.：BL1670A
Brand：Biosharp
Size：500 mL/bottle
Product Description
Biosharp BL1670A is a serum-free, low-osmolarity, optimized basal medium designed for long-term culture, maturation, and survival of primary embryonic/prenatal neurons (e.g., hippocampal, cortical, striatal). When combined with B-27 supplement, it supports robust neuronal survival without an astrocyte feeder layer白鲨.
Specifications
Form：Red, clear liquid; ready-to-use basal medium
Target Cells：Primary embryonic/prenatal rodent neurons (hippocampus, cortex, striatum, substantia nigra, etc.)
Required Supplements：B-27 (50×) recommended; N-2 also compatible
Free of：Serum, L-glutamine, L-glutamic acid, L-aspartic acid
Culture Conditions：37°C, 5% CO₂, humidified atmosphere
Storage：2–8°C, protected from light
Shelf Life：12 months
Key Features
Serum-Free & High Purity：Eliminates serum lot variation and contamination risks; yields highly homogeneous neuronal populations
Long-Term Maintenance & Maturation：Supports ≥2–4 weeks survival, neurite outgrowth, and synaptic maturation of primary neurons
Optimized & Low-Stress Formulation：Osmolarity and nutrient balance mimic the in vivo CNS microenvironment, reducing cellular stress and apoptosis
High Compatibility：Fully compatible with B-27/N-2 supplements; ideal for drug screening, neurotoxicology, electrophysiology, and molecular biology assays
Recommended Protocol (Simplified)
Basal Medium：BL1670A (500 mL)
Complete Medium：BL1670A + 2% B-27 (50×) + 0.5 mM L-glutamine
Plating：Add 25 μM L-glutamic acid during initial seeding for improved survival
Antibiotics：Use at half the standard concentration (B-27 lacks serum’s detoxifying effects)
Precautions
For research use only：Not for clinical diagnosis, therapy, or human administration
Use aseptic technique to avoid microbial contamination.
Do not freeze-thaw repeatedly; use within 1 month after opening.