CheKineTM 葡萄糖激酶 (GCK）活性检测试剂盒(微

CheKine™ Micro Glucokinase (GCK) Activity Assay Kit

专为精准细胞代谢研究设计的微量检测方案

货号	KTB1131	检测指标	葡萄糖激酶
样本类型	动植物组织、细胞、细菌、血清（浆）及其他生物体液
检测范围	15.625-1000 μM	灵敏度	15.625 μM
保存条件	-20℃避光保存(6个月)	运输方式	蓝冰冰袋运输

核心组分与检测原理

• 提取液
• 试剂一至试剂六
• 标准品

基于比色法检测原理，通过测量NADPH在340nm处的吸光度变化，定量分析GCK催化葡萄糖转化为葡萄糖-6-磷酸的活性。适用于微量样本分析需求。

关键科研应用

葡萄糖激酶(GCK)在肝细胞和胰岛β细胞中特异性表达，催化葡萄糖磷酸化反应(Km值约8mM)。该试剂盒适用于：

• 糖尿病发病机制研究
• 胰岛素分泌调控分析
• 糖代谢通路功能验证
• 药物靶点活性筛选

操作注意事项

• 需通过预实验确定最佳样本量
• 强烈建议采用BCA法进行样本均一化
• 每次实验需自行建立标准曲线
• 操作时需在通风橱内佩戴防护手套

产品规格及交付

规格	价格(RMB)	货期
48T/48S	2406	现货(次日发货)
96T/96S	3006	现货(次日发货)

安全警告：本品含具刺激性/毒性生化试剂，操作请遵循实验室安全规范

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Glucokinase (GCK) Activity Assay Kit

A micro-scale detection solution specifically designed for precise cellular metabolism research

Catalog No.	KTB1131	Target	Glucokinase
Sample Type	Animal/plant tissues, cells, bacteria, serum (plasma), and other biological fluids
Detection Range	15.625-1000 μM	Sensitivity	15.625 μM
Storage Conditions	Store at -20°C protected from light (6 months)	Shipping Method	Blue ice packs

Core Components and Detection Principle

• Extraction Buffer
• Reagent 1 to Reagent 6
• Standard

Based on the colorimetric detection principle, this kit quantitatively analyzes GCK activity by measuring NADPH absorbance changes at 340 nm during glucose-to-glucose-6-phosphate conversion. Suitable for micro-scale sample analysis requirements.

Key Research Applications

Glucokinase (GCK), specifically expressed in hepatocytes and pancreatic β-cells, catalyzes glucose phosphorylation (Km ≈8mM). This kit is applicable for:

• Diabetes pathogenesis studies
• Insulin secretion regulation analysis
• Glycometabolic pathway functional validation
• Drug target activity screening

Operation Notes

• Optimize sample loading through pre-experiments
• Strongly recommend BCA method for sample normalization
• Establish standard curves for each experiment
• Operate in fume hood with protective gloves

Specifications and Delivery

Specification	Price (RMB)	Lead Time
48T/48S	2406	In stock (shipped next day)
96T/96S	3006	In stock (shipped next day)

Safety Warning: Contains irritating/toxic biochemical reagents. Follow laboratory safety protocols.

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Note: Above information may change due to product optimization. For updated details, please contact AccuScience.