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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 目录编号:
112-065-175
- 库存:
详询
- 供应商:
广州市左克生物
- 浓度:
见包装
- 抗体英文名:
Biotin-SP-conjugated AffiniPure® Goat Anti-Rat IgG, Light Chain* Specific (min X Bov,Gt,Hrs,Hu,Ms,Rb,Shp Ig)
- 规格:
0.5ml
产品简介:
品牌:Jacksonimmuno
货号:112-065-175
产品名称:Biotin-SP-conjugated AffiniPure® Goat Anti-Rat IgG, Light Chain* Specific (min X Bov,Gt,Hrs,Hu,Ms,Rb,Shp Ig)
https://www.jacksonimmuno.com/catalog/products/112-065-175
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining
by staining in simple IHC the relevant heterologous primary antibodies. E.g. many goat anti mouse IgG1 heavy chain do stain rat IgG2a and IgG2b . Procedure: First stain HRP Developing solution Second stain AP
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