Alkaline Phosphatase-conjugate
d AffiniPure® Sheep Anti-Mouse IgG, F(ab')2 Fragment Specific (min X Hu,Bov,Hrs Sr Prot)- ￥2754
- Jacksonimmuno
- 美国
- 515-055-072
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 目录编号：
515-055-072
- 库存：
详询
- 供应商：
广州市左克生物
- 浓度：
见包装
- 抗体英文名：
Alkaline Phosphatase-conjugated AffiniPure® Sheep Anti-Mouse IgG, F(ab')2 Fragment Specific (min X Hu,Bov,Hrs Sr Prot)
- 规格：
1ml
产品简介:
品牌：Jacksonimmuno
货号：515-055-072
产品名称：Alkaline Phosphatase-conjugated AffiniPure® Sheep Anti-Mouse IgG, F(ab')2 Fragment Specific (min X Hu,Bov,Hrs Sr Prot)
https://www.jacksonimmuno.com/catalog/products/515-055-072
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文献和实验ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
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