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- 禾午生物
- P78543
- 国产
- 2025年08月03日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pCMV-Adar(mouse)-1-3×FLAG-Neo
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃＆负80℃
- 规格：
干粉＆甘油菌
| 名称 | pCMV-Adar(mouse)-1-3×FLAG-Neo |
| 别称 | NM_019655.3;Adar1; Adar1p110; Adar1p150; AV242451;DRADA; mZaADAR |
| 基因长 | 3456bp |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 小鼠 |
| 原核抗性 | Amp |
| 筛选标记 | Neo/G418 |
| 启动子 | CMV |
| 复制子 | pUC |
| 感受态 | DH5a |
| 温度 | 37度 |
| 正向引物 | CMV-F |
| pCI-3×FLAG-CVS-11-P(virus)-Neo |
| pCMV-Eef1a1(mouse)-3×Myc-Neo |
| pCMV-TMF1(human)-3×HA-Neo |
| pCMV-CPSF6(human)-3×HA-Neo |
| pCMV-Adar(mouse)-1-3×FLAG-Neo |
| pCMV-DLAT(human)-9-3×FLAG-Neo |
| pCMV-Cisd2(mouse)-3×Myc-Neo |
| pCMV-SMURF2(human)-3×HA-Neo |
| pCMV-SYVN1(human)-3×HA-Neo |
| pCMV-SOCS7(human-gc-opt)-3×HA-Neo |
| pCMV-HSPA9(human)-3×HA-Neo |
| pCMV-Tgif1(rat)-3×FLAG-Neo |
| pCMV-KLRC4(human)-3×FLAG-Neo |
| pCMV-TP53(human)-R248Q-3×FLAG-Neo |
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文献和实验and protein expression procedures for producing Drosophila ADAR in this system. ADAR expression in Pichia pastoris uses the methanol-inducible alcohol oxidase AOX1 promoter for induction. A Zeocin resistance gene on the plasmid is used to select
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
技术资料