- ￥1600
- Sciencell
- C-7501
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
MSCM (Mesenchymal Stem Cell Medium)
- 规格：
500ml/T
|
货号 |
7501 |
|
产地 |
美国 |
|
缩写 |
MSCM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
间充质干细胞培养基是专门为人类间充质干细胞体外培养所设计的、最适合其生长的培养基。是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(5%)。该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类微血管内皮细胞体外培养中的增殖和生长，并为其达到最理想营养平衡状态提供数量上和质量上的保证。
间充质干细胞培养基包含500 ml基础培养基，25ml胎牛血清(FBS,目录编号0025)，5ml间充质干细胞生长添加物，(MSCGS,目录编号7552)和5 ml青霉素/链霉素溶液(P/S,目录编号0503)
1.) Reed SA, Johnson SE. (2013) "Expression of scleraxis and tenascin C in equine adipose and umbilical cord blood derived stem cells is dependent upon substrata and FGF supplementation."?Cytotechnology. doi. 10.1007/s10616-012-9533-3.
2.) Johnson SP, Catania JM, Harman RJ, Jensen ED. (2012) "Adipose-derived stem cell collection and characterization in bottlenose dolphins (Tursiops truncatus)."?Stem Cells Dev. 21: 2949-57.?
3.) Ma Z, Gao BZ. (2012) "Quantitatively analyzing the protective effect of mesenchymal stem cells on cardiomyocytes in single-cell biochips."?Biotechnol Lett. 34: 1385-91.?
4.) Ma Z, Liu Q, Liu H, Yang H, Yun JX, Xu M, Eisenberg CA, Borg TK, Markwald R, Gao BZ. (2012) "Cardiogenic Regulation of Stem-Cell Electrical Properties in a Laser-Patterned Biochip."?Cell Mol Bioeng. 5: 327-336.?
5.) Panicker SP, Ganguly T, Consolo M, Price V, Mirmirani P, Honda K, Karnik P. (2012) "Sterol intermediates of cholesterol biosynthesis inhibit hair growth and trigger an innate immune response in cicatricial alopecia." PLoS One. 7: e38449.?
6.) Suh N, Paul S, Lee HJ, Yoon T, Shah N, Son AI, Reddi AH, Medici D, Sporn MB. (2012) "Synthetic triterpenoids, CDDO-Imidazolide and CDDO-Ethyl amide, induce chondrogenesis."?Osteoarthritis Cartilage. 20: 446-50.?
7.) Sun HP, Zhang X, Chen XH, Zhang C, Gao L, Feng YM, Peng XG, Gao L. (2012) "Human umbilical cord blood-derived stromal cells are superior to human umbilical cord blood-derived mesenchymal stem cells in inducing myeloid lineage differentiation in vitro."?Stem Cells Dev. 21: 1429-40.
8.) Wang Y, Gao R, Wang PP, Jian J, Jiang XL, Yan C, Lin X, Wu L, Chen GQ, Wu Q. (2012) "The differential effects of aligned electrospun PHBHHx fibers on adipogenic and osteogenic potential of MSCs through the regulation of PPAR? signaling."?Biomaterials. 33: 485-93.?
9.) Choi KS, Ahn SY, Kim TS, Kim J, Kim BG, Han KH, Ban SJ, Kim HS, Choi Y, Lim CJ. (2011) "Characterization and biodistribution of human mesenchymal stem cells transduced with lentiviral-mediated BMP2."?Arch Pharm Res. 34: 599-606.?
10.) Dissanayaka WL, Zhu X, Zhang C, Jin L. (2011) "Characterization of dental pulp stem cells isolated from canine premolars."?J Endod. 37: 1074-80.?
11.) Zhao Z, Chen Z, Zhao X, Pan F, Cai M, Wang T, Zhang H, Lu JR, Lei M. (2011) "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions."?J Biomed Sci. 18: 37.
12.) Gong Z, Calkins G, Cheng EC, Krause D, Niklason LE. (2009) "Influence of culture medium on smooth muscle cell differentiation from human bone marrow-derived mesenchymal stem cells."?Tissue Eng Part A. 15: 319-30.
13.) Reed SA, Johnson SE. (2008) "Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types."?J Cell Physiol. 215: 329-36.?
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文献和实验
1.) Reed SA, Johnson SE. (2013) "Expression of scleraxis and tenascin C in equine adipose and umbilical cord blood derived stem cells is dependent upon substrata and FGF supplementation."?Cytotechnology. doi. 10.1007/s10616-012-9533-3.
2.) Johnson SP, Catania JM, Harman RJ, Jensen ED. (2012) "Adipose-derived stem cell collection and characterization in bottlenose dolphins (Tursiops truncatus)."?Stem Cells Dev. 21: 2949-57.?
3.) Ma Z, Gao BZ. (2012) "Quantitatively analyzing the protective effect of mesenchymal stem cells on cardiomyocytes in single-cell biochips."?Biotechnol Lett. 34: 1385-91.?
4.) Ma Z, Liu Q, Liu H, Yang H, Yun JX, Xu M, Eisenberg CA, Borg TK, Markwald R, Gao BZ. (2012) "Cardiogenic Regulation of Stem-Cell Electrical Properties in a Laser-Patterned Biochip."?Cell Mol Bioeng. 5: 327-336.?
5.) Panicker SP, Ganguly T, Consolo M, Price V, Mirmirani P, Honda K, Karnik P. (2012) "Sterol intermediates of cholesterol biosynthesis inhibit hair growth and trigger an innate immune response in cicatricial alopecia." PLoS One. 7: e38449.?
6.) Suh N, Paul S, Lee HJ, Yoon T, Shah N, Son AI, Reddi AH, Medici D, Sporn MB. (2012) "Synthetic triterpenoids, CDDO-Imidazolide and CDDO-Ethyl amide, induce chondrogenesis."?Osteoarthritis Cartilage. 20: 446-50.?
7.) Sun HP, Zhang X, Chen XH, Zhang C, Gao L, Feng YM, Peng XG, Gao L. (2012) "Human umbilical cord blood-derived stromal cells are superior to human umbilical cord blood-derived mesenchymal stem cells in inducing myeloid lineage differentiation in vitro."?Stem Cells Dev. 21: 1429-40.
8.) Wang Y, Gao R, Wang PP, Jian J, Jiang XL, Yan C, Lin X, Wu L, Chen GQ, Wu Q. (2012) "The differential effects of aligned electrospun PHBHHx fibers on adipogenic and osteogenic potential of MSCs through the regulation of PPAR? signaling."?Biomaterials. 33: 485-93.?
9.) Choi KS, Ahn SY, Kim TS, Kim J, Kim BG, Han KH, Ban SJ, Kim HS, Choi Y, Lim CJ. (2011) "Characterization and biodistribution of human mesenchymal stem cells transduced with lentiviral-mediated BMP2."?Arch Pharm Res. 34: 599-606.?
10.) Dissanayaka WL, Zhu X, Zhang C, Jin L. (2011) "Characterization of dental pulp stem cells isolated from canine premolars."?J Endod. 37: 1074-80.?
11.) Zhao Z, Chen Z, Zhao X, Pan F, Cai M, Wang T, Zhang H, Lu JR, Lei M. (2011) "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions."?J Biomed Sci. 18: 37.
12.) Gong Z, Calkins G, Cheng EC, Krause D, Niklason LE. (2009) "Influence of culture medium on smooth muscle cell differentiation from human bone marrow-derived mesenchymal stem cells."?Tissue Eng Part A. 15: 319-30.
13.) Reed SA, Johnson SE. (2008) "Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types."?J Cell Physiol. 215: 329-36.?
PromoCell MSC分化培养操作步骤 一、向神经细胞分化的操作流程 1、将塑料培养器用纤维连接蛋白包被根据操作手册，用纤维连接蛋白（C-43050）包被合适的组织培养瓶。使用浓度为10μg/ml。2、接种间充质干细胞用MSC生长培养基（C-28010）在用纤维连接蛋白包被过的培养瓶中接种培养，接种密度为4000个/cm2。3、培养间充质干细胞将细胞培养至80%的饱和度，每48小时更换一次培养基。4、诱导间充质干细胞用MSCU经细胞分化培养基（C-28015）诱导培养，用MSC
实验大纲 骨髓间充质干细胞是多能干细胞。因此，作为再生医学的细胞来源，它是活性研究和临床应用的目标。当骨髓液或骨髓细胞接种在培养皿上时，间充质干细胞会作为粘附细胞增殖。因此，间充质干细胞可以从漂浮的血细胞中分离出来。在此，我们报告了使用 Evident Prov CM20 培养监测系统对间充质干细胞原代培养的远程监测结果。 试验程序 使用补充了 10% 胎牛血清（FBS）和 1% 青霉素链霉素的 Iscove 改良 Dulbecco 培养基（IMDM），将市售人骨髓细胞以 950,000
原代细胞分离和培养基本步骤1、器官和组织的选择尽可能的去除不必要的组织和血迹。2、原代细胞的分离（1）应用PriCells原代细胞分离试剂盒I、II、III。（2）根据组织来源不同，可选用不同的PriCells原代细胞分离试剂盒。3、原代细胞的培养：（1）PriCells原代细胞特制培养基（2）PriCells原代细胞特制添加物（3）优质胎牛血清4、细胞的培养和鉴定：PriCells原代细胞鉴定试剂盒5、原代细胞的传代、保存（1）传代：依椐细胞的生长状态，生长的细胞1：2或1：3进行传代
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