原代成纤维细胞培养基

货号	2301
产地	美国
缩写	FM
规格	500ml
用途	科研
储存	4度，-20度
运输	胶冰

成纤维细胞培养基是为正常人类成纤维细胞体外培养设计的适于其生长的培养基。它是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为HEPES和重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类成纤维细胞体外培养中的增殖和生长，并为其达到理想营养平衡状态提供数量上和质量上的保证。

 

成纤维细胞培养基包含500 ml基础培养基，10ml胎牛血清(FBS,目录编号0010)，5ml成纤维细胞生长添加物(FGS,目录编号2352)和5 ml青霉素/链霉素溶液(P/S,目录编号0503)

1.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) 'Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer.'

 

2.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) "Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer." PloS one 9: e96878.

 

3.) Li F, Weir MD, Chen J, Xu HHK. (2013) "Comparison of quaternary ammonium-containing with nano-silver-containing adhesive in antibacterial properties and cytotoxicity."Dent Mater. 29: 450-61.

 

4.) Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.

 

5.) Zhang K, Cheng L, Imazato S, Antonucci JM, Lin NJ, Lin-Gibson S, Bai Y, Xu HHK. (2013) "Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties." J Dent. 41: 464-74.

 

6.) Cavet ME, Volhejn S, Harrington KL, Zhang JZ. (2013) "Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells." Mol Vis. 19: 1515-25.

 

7.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) 'TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression.'

 

8.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) "TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression." PloS one. 8: e77366.

 

9.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'

 

10.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) 'B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts.'

 

11.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.

 

12.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) "Export of microRNAs and microRNA-protective protein by mammalian cells." Nucleic Acids Res. 38:7248-59.