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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at RT, 1 year
- 保质期:
Store at RT, 1 year
- 英文名:
10×SDS-PAGE Fast Running Buffer(Tris-Gly, Powder)
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
1L
SDS-PAGE Fast Running Buffer is an improved SDS-PAGE fast running buffer based on the conventional Tris-Gly buffer system. This solution has the following advantages compared to traditional running buffer: ① It can perform constant pressure running at 200V, with less heat generation and low temperature; ② Significantly improve running efficiency, shorten running time, and complete SDS-PAGE protein running in about 30 minutes; ③ The obtained protein bands are sharp and clear, without dragging; ④ Good separation effect for small molecular weight proteins; ⑤ It is suitable for fast running of conventional dye free gel or premix (suitable for Tris-Gly running) with different concentrations, and has better separation effect for low concentration gels such as 6%, 8% and 10%.
This product is a pre mixed dry powder of 10×SDS-PAGE Fast Running Buffer, which is easy to store and operate. It needs to be dissolved in distilled water before use. Each bag of powder can be prepared into 10L of 1×SDS-PAGE Fast Running Buffer.
| 基本信息 | |
| 基因名 | |
| 英文名称 | 10×SDS-PAGE Fast Running Buffer(Tris-Gly, Powder) |
| 中文名称 | 10×SDS-PAGE快速电泳液(Tris-Gly, 干粉) |
| 外观(性状) | 白色粉末 |
| 储存条件 | Store at RT, 1 year |
| 运输条件 | 室温运输 |
| 单位 | 袋 |
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文献和实验Although the dopamine hypothesis of schizophrenia has gained wide acceptance, it has recently become clear that it requires revision (Seeman and Niznik, 1990 ; Ellison, 1994 ; Kahn and Davis, 1995 ). The original hypothesis asserts
一、TBS的配制 0.05 M TBS ( pH7.4 ) 的配制: Tris ( 三羟甲基胺基甲烷 ) 12.1g NaCl 17.5g 加蒸馏水 1500ml 磁性搅拌下滴加浓HCl至pH为7.4,再加蒸馏水至2000ml,即可。 如需含1% Triton X-100,则在滴加HCl前先加入20
的1MOL/ML的Tris(PH8.0),反复混匀后,静至分层;放出下层黄色酚液,弃上层 4、加固体Tris约1g/100ml酚,摇匀,去水相 5、加0.1MOL/MLTris(PH8.0)平衡数次,至PH为8.0 6、加0.1MOL/MLTris(PH8.0)于棕色瓶中4度保存 7、如黄色消失或呈粉红色,则不能使用
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