相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
100test/25test
| 规格: | 100test | 产品价格: | ¥3199.0 |
|---|---|---|---|
| 规格: | 25test | 产品价格: | ¥1063.0 |
产品简介:
品牌:MultiSciences/联科生物
货号:70-KTR201-100///70-KTR201-25
产品简介
调节性T细胞(Tregs),曾被称为抑制性T细胞,是T细胞的亚群,可调节免疫系统、维持对自身抗原的耐受、抑制自身免疫疾病。Tregs具有免疫抑制性,可抑制或下调效应T细胞的诱导和增殖。调节性T细胞有多种类型,其中研究最深的的一类表达CD4、CD25和FOXP3(CD4+CD25+调节性T细胞)。免疫系统必须能区分自我和非我。当自我/非我无法识别时,免疫系统会破坏细胞和组织,引起自身免疫疾病。调节性T细胞积极地抑制免疫系统活化、阻止病理性的自身反应如自身免疫疾病。调节性T细胞遗传缺失造成的重度自身免疫综合征(IPEX综合征)表明调节性T细胞在免疫系统中发挥了重要作用。
产品特点
- 即用型,无需购买其他产品;
- 灵敏度高,特异性强,结果稳定;
- 价格便宜,现货供应。
结果示例

Flow cytometric analysis of Mouse Regulatory T Cell Staining Kit. The staining pattern of FSC/CD4 (Left) CD25/Mouse IgG1 Isotype Control, PE (Middle) and CD25/FoxP3 (Right) on splenocytes of normal ICR mouse. Adherent cells have been excluded. Flow cytometry was performed on a Thermo Fisher Attune NxT.
使用Mouse Regulatory T Cell Staining Kit进行流式检测。正常ICR小鼠的脾细胞染色FSC/CD4 (左) CD25/小鼠IgG1同型对照, PE (中)和CD25/FoxP3(右)。粘连细胞已被排除。实验在Thermo Fisher公司的Attune NxT流式细胞仪上进行。
试剂盒组分
| 组分 | 编号 | 25Test | 100Test |
| 抗小鼠CD4, FITC (克隆号:GK1.5) | AM00401 | 150 μl | 600 μl |
| 抗小鼠 CD25, APC (克隆号:PC61.5) | AM02505 | 150 μl | 600 μl |
| 抗小鼠FoxP3, PE (克隆号:3G3) | AM0F04 | 150 μl | 600 μl |
| 抗小鼠CD16/CD32, 纯化 (克隆号:2.4G2) | AM016 | 60 μl | 240 μl |
| 小鼠IgG1同型对照, PE | CMG104 | 25 μl | 100 μl |
| 固定破膜剂浓缩液(4×) | ICC01 | 8 ml | 30 ml |
| 固定破膜剂稀释液 | ICD01 | 25 ml | 100 ml |
| 破膜缓冲液(10×) | ICB01 | 25 ml | 100 ml |
| 红细胞裂解液(不含固定剂) (10×) | LYS | 8 ml | 30 ml |
| 流式染色缓冲液(10×) | S1002 | 15 ml | 60 ml |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Regulatory T Cell Enrichment by IFN- Conditioning
IFN-γ was originally characterized as a proinflammatory cytokine with T helper type 1 inducing activity, but it is now clear that it also has important immunoregulatory functions. Regulatory T cells play an important role in models
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Generation of T Cell Hybridomas from Naturally Occurring FoxP3+ Regulatory T Cells
Generation of regulatory T cells (or Treg) derived hybridomas offers a tool to study their antigen specificity. T cells hybridomas are produced by fusing TCR α-β-thymoma BW5147 with highly dividing T cell population. In vitro anergy of Tregs
技术资料暂无技术资料 索取技术资料







