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- P00269
- 2026年08月13日
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- 文献和实验
- 技术资料
- 保质期：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br> -70°C for 3 months under sterile conditions after redissolves. <br> ≤-70°C, stable for 6 months after receipt.
- 保存条件：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br> -70°C for 3 months under sterile conditions after redissolves. <br> ≤-70°C, stable for 6 months after receipt.
- 英文名：
2019-nCoV NP NTD domain
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/50ug/500ug
| 规格： | 1mg | 产品价格： | ¥5325 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ¥606 |
| 规格： | 500ug | 产品价格： | ¥3505 |
| 基因名 | 2019-nCoV NP NTD domain |
| 别名 | 2019-nCoV NP NTD domain |
| 蛋白号 | QHD43423.2 |
| 种属 | 2019-nCoV |
| 宿主 | E.coli |
| 产品描述 | Recombinant SARS-CoV-2 NP NTD domain is produced by our E.coli expression system with a 6His tag at the N-terminus. |
| 区间范围 | NA |
| 标签信息 | N-6His |
| 预测分子量 | 15.9kDa |
| 表观分子量 | 16kDa, reducing conditions |
| 纯度 | ≥95% by SDS‐PAGE. |
| 内毒素 | Not measured |
| 溶剂 | Supplied as a 0.2 μm filtered solution of PBS, 2M Urea, pH 7.4 |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -70°C for 3 months under sterile conditions after redissolves. ≤-70°C, stable for 6 months after receipt. |
| 运输条件 | 干冰运输（需要收取干冰费） |
| 背景说明 | Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | No activity experimental data, to be verified. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 生物制药相关研究 |
| 英文名称 | 2019-nCoV NP NTD domain |
| 中文名称 | 2019-nCoV NP NTD domain蛋白 |
| 单位 | 支 |
| 规格 | 1mg ; 50ug ; 500ug |
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文献和实验常用1%以下的中性去垢剂，如Tween、Triton、Lubel和 NP40等加EDTA和还原剂2-巯基苏糖醇（DTT）、β-巯基乙醇等反复多次进行，因去垢剂洗涤能力随溶液离子强度升高而加强，在洗涤包涵体时可加50 mM NaCL。这样提取的包涵体纯度至少可达50%以上，而且可保持元结构。也可用低浓度的盐酸胍或尿素/中性去垢剂/EDTA/还原剂等洗去包涵体表面吸附的大部分不溶性杂蛋白。洗涤液pH以与工程菌生理条件相近为宜，使用的还原剂为0.1-5mM。EDTA为0.1-0.3 mM。去垢
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