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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
1mg/500ug/50ug/10ug
| 规格: | 1mg | 产品价格: | ¥9120.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥6240.0 |
| 规格: | 50ug | 产品价格: | ¥936.0 |
| 规格: | 10ug | 产品价格: | ¥312.0 |
| 基本信息 | |
| 基因名 | |
| 别名 | NEDD8-conjugating enzyme Ubc12;NEDD8 carrier protein;NEDD8 protein ligase;Ubiquitin-conjugating enzyme E2 M;UBC12;UBE2M; |
| 中文名称 | Recombinant Human UBE2M/NEDD8-conjugating enzyme Ubc12/UBC12 |
| 外观(性状) | Supplied as a 0.2 μm filtered solution of 50mM HEPES,pH 7.5,2mM DTT,150mM NaCl,10% Glycerol. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | 干冰运输 |
| 背景说明 | UBE2M is a member of the E2 ubiquitin-conjugating enzyme family.Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes,or E1s,ubiquitin-conjugating enzymes,or E2s,and ubiquitin-protein ligases,or E3s.This protein is linked with a ubiquitin-like protein,NEDD8,which can be conjugated to cellular proteins,such as Cdc53/culin.UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins.The specific interaction with the E3 ubiquitin ligase RBX1,but not RBX2,suggests that the RBX1-UBE2M complex neddylates specific target proteins,such as CUL1,CUL2,CUL3 and CUL4.It involved in cell proliferation and is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. |
| 来源 | E.coli |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验Studies of the Ubiquitin Proteasome System
( protocol 34 ) E2‐UbcH5B ( protocol 37 ) GST‐ubiquitin ( protocol 29 ) Human APP‐BP1/Uba3 (NEDD8 E1; protocol 21 ) Ubc12 (E2
在无血清细胞培养条件下将人 iPS 细胞体外分化为结肠类器官
)中。 4 °C过夜或在冰上解冻足量的减生长因子的ECM基质胶(E6909)。 在结肠上皮细胞分化的第 12 天,从培养箱中取出细胞并将漂浮在培养基中的球状体收集到 15 mL 锥形管中。 用 3 mL 1X PBS 清洗每个孔。 用 1 mL Accumax (A7089) 解离含有球状体(非悬浮球状体)的单层细胞,并37 °C孵育 5-10 分钟。 解离单层后,用 P-1000 移液器轻轻移取细胞 4-5 次,然后将 1 mL 细胞悬液转移到上述步骤 9 中制备的锥形管中。 4 °
。[11][12][13]真核生物的核糖核酸酶P中的RNA组分直到最近才被证明是核酶;[14]其蛋白质亚基自身对于底物tRNA的剪切只具有次要作用,[15]这些亚基可能是在核糖核酸酶P和核糖核酸酶MRP的其他功能(如基因转录和细胞周期)中发挥主要作用。[4][16] 参考文献 ^ 1.0 1.1 (英文)Guerrier-Takada C, Gardiner K, Marsh T, Pace N, Altman S. The RNA moiety of ribonuclease
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