P00208 Recombinant Mouse S100

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标题:Enteric glial cells aggravate the intestinal epithelial barrier damage by secreting S100汕 under high-altitude conditions

成员:Xie Huichao, Zeng Xiong, Wang Wensheng, Wang Wei, Han Ben, Tan QianShan, Hu Qiu, Liu Xingyu, Chen Shuaishuai, Chen Jun, Sun Lihua, Chen Yihui, Xiao Weidong

论文因子:4 发表期刊:Molecular Biomedicine pmid:37779161

Recombinant Mouse S100 Calcium Binding Protein B is produced by our E.coli expression system and the target gene encoding Met1-Glu92 is expressed with a 6His tag at the C-terminus.

基本信息
基因名
别名	Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100b;S100 beta;S100 calcium binding protein B
外观(性状)	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
纯度	≥95% as determined by reducing SDS-PAGE.
储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输条件	The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below.
背景说明	S100-B，is an acidic protein with a molecular weight of 21 kDa belonging to the S100 family.S100-B contains two EF-hand-type calcium-binding motifs separated by a hinge region with a hydrophobic cleft.S100-B plays an important role in neurodevelopment，differentiation，and brain construction.S100-B has neuroprotective effects，but at high concentrations S100-B is neurotoxic.Extracellular concentration of S100-B increases following brain damage，which easily penetrates into cerebrospinal fluid in brain damage and then into the blood.S100-B is expressed and produced by astrocytes in vertebrate brains and in the CNS，and the astrocytes are the major cells producing S100-B protein in gray matter，as well as oligodendrocytes are the predominant S100-B in protein producing cells in white matter.The major advantage of using S100-B is that elevations in serum or CSF levels provide a sensitive measure for determining CNS injury at the molecular level before gross changes develop，enabling timely delivery of crucial medical intervention before irreversible damage occurs.In addition，S100-B，which is also present in Mouse melanocytes，is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.
来源	E.coli
溶剂	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
内毒素	Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test.
单位	支