青蒿琥酯

使用本产品的案例（仅供参考）

In Vitro

Cell（ARPE-19，0~200µM Artesunate,0~72h）

The cells were cultured to 80% − 90% fusion degree, starved overnight in serum-free medium, and replaced with DMEM / F12 containing 1% FBS,Different concentrations of artesunate (Solarbio) (0,50,100,150,200µM)Cell proliferation and migration were detected at different time points (0, 24, 48 and 72h).   TGF was used-βThe degree of EMT was detected after 48 hours of pretreatment with artesunate.

来源文献：Wang ZY, Zhang Y, Chen J, Wu LD, Chen ML, Chen CM, Xu QH. Artesunate inhibits the development of PVR by suppressing the TGF-β/Smad signaling pathway. Exp Eye Res. 2021 Dec;213:108859. doi: 10.1016/j.exer.2021.108859. Epub 2021 Nov 23. PMID: 34822854.

In Vivo

Mice（Young (4 month) mice：30µg/g Artesunate，1次/天，16天，口服；Aged (20 month) mice：30µg/g Artesunate，1次/2天，口服）

Young (4 month) mice were randomly divided into 3 groups (n=5): Vehicle, Tm and Tm+Artesunate. The mice were orally administrated with Artesunate (Solarbio, China, 30 µg/g, dissolved in 5 % NaHCO3) for successive 16 days.    Body weight was measured every day. Tm (6 μg/g) was intraperitoneally administrated 15 h before sacrifice.    FD-4 was administrated 3 h before sacrifice for the evaluation of intestinal permeability. Colon length was measured and mucosa tissues were removed for subsequent experiments.

Aged (20 month) mice were randomly divided into 2 groups (n=5): Vehicle and Artesunate. The mice were orally administrated with Artesunate (30 µg/g) every other day for a total of 25 doses.    Body weight was measured every 2 days. Length of colon was measured at sampling time and FD-4 leakage were measured. Colon mucosa tissues were removed for subsequent experiments.

来源文献：Chen H, Sun HM, Wu B, Sun TY, Han LZ, Wang G, Shang YF, Yang S, Zhou DS. Artesunate delays the dysfunction of age-related intestinal epithelial barrier by mitigating endoplasmic reticulum stress/unfolded protein response. Mech Ageing Dev. 2023 Mar;210:111760. doi: 10.1016/j.mad.2022.111760. Epub 2022 Dec 5. PMID: 36476344.

Rabbit（2-2.5kg Adult pigmented rabbits, 20µg/mL Artesunate, 注射于玻璃体）

Twelve adult pigmented rabbits were selected, each weighing about 2-2.5kg, After extracted 0.2ml vitreous,12 traumatic PVR model rabbits were prepared by intravitreal injection of 0.1ml platelet rich plasma and 0.1mlPBS.   They were randomly divided into blank group, control group (0.1mlprp +0.1mlpbs) and experimental group: (0.1mlPRP + 0.1mL 20µg/ml artesunate).    In the control group, 0.1mL platelet-rich plasma and 0.1mL PBS were injected into the vitreous cavity at the same time. In the experimental group, 0.1mL platelet rich plasma and 0.1ml artesunate (20µg/ml) were injected into the vitreous cavity at the same time. Fundus photography and B ultrasound machine were used to observe the proliferation of the vitreous and retina at different periods. The expression of Vim, Smad3 and PSmad3 in vitreous retinal hyperplasia was detected by Western blotting 28 days after surgery, and the retinal tissue structure of each group was observed by frozen section.

来源文献：Wang ZY, Zhang Y, Chen J, Wu LD, Chen ML, Chen CM, Xu QH. Artesunate inhibits the development of PVR by suppressing the TGF-β/Smad signaling pathway. Exp Eye Res. 2021 Dec;213:108859. doi: 10.1016/j.exer.2021.108859. Epub 2021 Nov 23. PMID: 34822854.

Chen H, et al. Mech Ageing Dev. 2023 Mar;210111760

Wang ZY, et al. Exp Eye Res. 2021 Dec;213108859.