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- Solarbio
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- IA13001
- 2025年07月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
Artesunate(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
88495-63-0
- 规格：
0.1ml/0.3ml/0.5ml/1.5ml/1ml
| 规格： | 0.1ml | 产品价格： | ￥236.0 |
|---|---|---|---|
| 规格： | 0.3ml | 产品价格： | ￥354.0 |
| 规格： | 0.5ml | 产品价格： | ￥472.0 |
| 规格： | 1.5ml | 产品价格： | ￥767.0 |
| 规格： | 1ml | 产品价格： | ￥590.0 |
| 基本信息 | |
| CAS | No.88495-63-0 |
| 中文名称 | 青蒿琥酯(10mM in DMSO,无菌） |
| 英文名称 | Artesunate(10mM in DMSO,Sterile） |
| 分子式 | C19H28O8 |
| 分子量 | 384.42 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | STAT3;exported protein 1 (EXP1) |
| 通路 | JAK/STAT Signaling |
| 背景说明 | Artesunate是 STAT-3 和EXP1 的抑制剂。 |
| 生物活性 | Artesunate is an inhibitor of both STAT-3 and exported protein 1 (EXP1).[1-3] |
| In Vitro | Artesunate is an inhibitor of both STAT-3[1] and exported protein 1 (EXP1)[2]. Artesunate treatment for 24 h causes a significant increase in the levels of reactive oxygen species (ROS) in a dose-dependent manner in both cell lines. Moreover， Western blotting shows that the levels ofγ-H2AX are significantly elevated when cancer cells are treated with Artesunate in the higher dose range for 24 h. Artesunate also shows a time-dependent effect on the level of RAD51 in A2780 and HO8910 cells. In two types of non-malignant cells， normal human fibroblasts and immortalized epithelial cells， FTE-187， the level of RAD51 is not altered by Artesunate. In A2780 cells， the level of RAD51 mRNA is indeed decreased by the addition of Artesunate， in a dose-dependent manner. Correspondingly， the promoter activity of RAD51 is significantly inhibited by Artesunate. In contrast， the RAD51 mRNA level in H8910 cells is not affected by Artesunate[3]. |
| 细胞实验 | Tumor growth is significantly reduced in the group receiving combined treatment of Artesunate and cisplatin (P[3]. |
| 细胞实验 | A2780 and HO8910 cells are cultured in RPMI 1640， Normal human fibroblasts (NHF) in DMEM， and FTE-187 in M199， supplemented with 10% fetal bovine serum， 100 units/mL penicillin， and 100 mg/mL streptomycin. All the cells are incubated in a humidified atmosphere of 95% air and 5% CO2. Artesunate is applied to the cultured cells at the concentration of 0， 5， 10， 25， or 50 μg/mL for various periods. The reactive oxygen species (ROS) production following Artesunate treatment is determined. Briefly， cells are loaded with 5 μM of CM-H2DCFDA and incubated at 37°C for 20 min after treatment with Artesunate. Cells are resuspended using preserving fluid and analyzed with a FACSCanto II. The peak excitation wavelength for oxidized CM-H2DCFDA is 490 nm and emission is 530 nm[3]. |
| 动物实验 | Four to six weeks old female athymic nude mice (BALB/c， nu/nu) are used. A2780 and HO8910 cells are harvested and resuspended in 0.1 ml of PBS， 5×106 cells/0.2 mL are injected subcutaneously into the left inguinal area of the mice. Two weeks later， mice bearing tumors (~70 mm3 for A2780 and HO8910) are randomly divided into 4 groups. Artesunate is administered daily via i.p. injection at doses of 50 mg/kg alone for 16 days. The tumor growth is monitored every other day. Tumor volume is determined by the formula 1/2a×b2 where a is the long diameter (mm) and b is the short diameter (mm)[3]. |
| 激酶实验 | After treatment with Artesunate for 24 h， cells are harvested and lysed in 1×cell lysis buffer. Total proteins of 15 to 25 μg are separated by SDS-PAGE and transferred to polyvinylidenedifluoride (PVDF) membranes. Membranes are blocked with 5% non-fat milk for 1 to 2 h at room temperature and then probed with primary antibodies and incubated at 4°C overnight. After extensive washing with TBS-T， membranes are incubated with appropriate HRP-conjugated secondary antibody for 1 h at room temperature， and then are detected by Western ECL-enhanced luminol reagent [3]. |
| 数据来源文献 | [1]. Ilamathi M， et al. Artesunate as an Anti-Cancer Agent Targets Stat-3 and Favorably Suppresses Hepatocellular Carcinoma. Curr Top Med Chem. 2016;16(22):2453-63. [2]. Lisewski AM， et al. Supergenomic network compression and the discovery of EXP1 as a glutathione transferase inhibited by artesunate. Cell. 2014 Aug 14;158(4):916-928. [3]. Wang B， et al. Artesunate sensitizes ovarian cancer cells to cisplatin by downregulating RAD51. Cancer Biol Ther. 2015;16(10):1548-56. |
| 单位 | 瓶 |
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IA13001 青蒿琥酯(10mM in DMSO,无菌） 抑制剂/拮抗剂/激动剂 索莱宝
￥236 - 767
