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- 询价记录
- 文献和实验
- 技术资料
- 库存:
大量
- CAS号:
点击查看
- 保质期:
有效期1年
- 保存条件:
2~8℃保存,一年有效。室温保存,3个月有效。
- 英文名:
Red Blood Cell Lysis Buffer 红细胞裂解液
- 供应商:
翌圣生物
- 规格:
100 mL(40401ES60)/250 mL(40401ES72)/500 mL(40401ES76)
| 规格: | 100 mL(40401ES60) | 产品价格: | ¥135 |
|---|---|---|---|
| 规格: | 250 mL(40401ES72) | 产品价格: | ¥255 |
| 规格: | 500 mL(40401ES76) | 产品价格: | ¥375 |
产品描述:
本产品采用了一种简便易行的方式,经配方优化保证既不破坏白细胞又能充分的将红细胞裂解去除,适用于人,大小鼠等哺乳动物新鲜血液。通常情况下,可以获得血液样品中全部白细胞的 80% 以上并充分保证白细胞的生物活性。
本产品主要成分是氯化铵,为即用型溶液,能快速、有效的裂解红细胞,富集分离白细胞。获得的白细胞生物活性好,可直接应用于流式细胞术、核酸或蛋白的提取、PCR等下游实验。
产品特色:
应用案例:
使用前先将低温保存红细胞裂解液平衡至室温。然后在血液样品中加入3倍体积红细胞裂解液 (例如,300 μL血液加入900 μL红细胞裂解液),颠倒混匀,室温放置5 min,期间再颠倒混匀几次 ,10000 rpm离心1 min (若是离心机较高转速不允许,可以3000 rpm离心5 min),吸去上清。用适当的缓冲液(如PBS, pH 7.2~7.4)清洗一遍。离心后留下白细胞沉淀进行后续的操作。

储存条件:
2~8℃保存,一年有效。室温保存,3个月有效。
常见问题:
Q:这款试剂做流式,对检测血液中的 CTC 有影响么?
A:没有对这个CTC 的测试数据。
Q:我们公司的 ACK 裂解红细胞后是否会对淋巴细胞RNA 有影响?
A:可用于后续核酸提取、PCR 等下游实验。
Q:红细胞裂解液中含有甲醛吗?
A:不含有。
Q:这款试剂是否为无菌?
A:无菌。
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Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis
) from erythrocytes and �granulocytes. However, this method is difficult to standardize especially under GMP conditions. Here, we describe an alternative approach for isolation of human MSC based on red blood cell (RBC) lysis with ammonium chloride. This results
红血球 erythrocyte,red blood corpuscle,red blood cell
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细胞裂解液(Cell Lysis Solution)的配置方法
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