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- 2025年07月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
Panobinostat(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
404950-80-7
- 规格：
1ml
| 基本信息 | |
| CAS | No.404950-80-7 |
| 中文名称 | 帕比司他(10mM in DMSO,无菌） |
| 英文名称 | Panobinostat(10mM in DMSO,Sterile） |
| 分子式 | C21H23N3O2 |
| 分子量 | 349.43 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | HDAC (Histone deacetylases) |
| 通路 | DNA Damage/DNA Repair;Epigenetics;NF-κB |
| 背景说明 | Panobinostat是一种非选择性组蛋白去乙酰化酶 (HDAC) 抑制剂。 |
| 生物活性 | Panobinostat (LBH589; NVP-LBH589) is a potent and orally active non-selective HDAC inhibitor， and has antineoplastic activities. Panobinostat induces HIV-1 virus production even at low concentration range 8-31 nM， stimulates HIV-1 expression in latently infected cells. Panobinostat induces cell apoptosis and autophagy. Panobinostat can be used for the study of refractory or relapsed multiple myeloma.[1-5] |
| In Vitro | Panobinosta (LBH589) induces apoptosis of both MOLT-4 and Reh cells in a time- and dose-dependent manner. Panobinosta treatment results in histone (H3K9 and H4K8) hyperacetylation and regulation of cell-cycle control genes in Reh cells[1]. Panobinostat exhibites potent antiproliferative activity in human NSCLC cell lines with the IC50 ranging from 5 to 100 nM[2].Panobinostat |
| 细胞实验 | Panobinosta (10， 20 mg/kg， i.p.) significantly slows tumor growth derived from Meso and NSCLC cells in vivo models. Panobinosta markedly increases acetylation of histone H3 and H4 of H69 human SCLC cells harvest from SCID mice[2]. Panobinostat (5， 10 and 20 mg/kg i.p.) demonstrates a clear benefit of decreased tumor burden， significantly improves TTE and reduces bone density loss in a disseminated multiple myeloma mouse model[3]. |
| 细胞实验 | Cells are washed with ice-cold PBS containing 0.1 mM sodium orthovanadate， and total proteins are isolated using RIPA lysis buffer， which includes protease inhibitors (leupeptin， antipain， and aprotinin)， 0.5 mM PMSF， and 0.2 mM sodium orthovanadate. Protein amounts are quantified using the Bio-Rad protein assay. Equal amounts of proteins are loaded onto an sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel， transferred onto nitrocellulose membrane， and probed with the antibody of interest: mouse monoclonal c-Myc and mouse monoclonal p21 antibodies; rabbit polyclonal phospho-Histone H2A.X， rabbit polyclonal acetyl-Histone H3 (Lys9)， and rabbit polyclonal acetyl-Histone H4 (Lys8) antibodies; mouse monoclonal p27/KIP1 antibody; mouse monoclonal anti-β-actin; and mouse monoclonal anti-GADD45G. Membranes are then washed， reprobed with appropriate horseradish peroxidase-conjugated secondary antibodies， and developed with SuperSignal chemiluminescent substrate.[1-5] |
| 动物实验 | AE17 and TC-1 cancer cells (1×106 cells) are injected into the flanks of adult female C57Bl/6 mice and severe combined immunodeficiency (SCID) mice. M30 (10×106 cells)， A549 (5×106 cells)， H69 (2.5×106 cells)， BK-T (6.5×106)， H526 (10×106)， and RG1 (10×106) cells are also injected， but in the presence of matrigel， into the flanks of SCID mice. When tumors reach 100 to 500 mm3， panobinostat is administered via i.p. injections (10-20 mg/kg) on a daily schedule (5-days-on， 2-days-off regimen) for the entire duration of the experiment. Control micereceive i.p. injections with dextrose 5% in water. Every tumor is measured with a caliper at least twice weekly. For evaluation of the effects of combination therapy on SCLC-derived tumors， SCID mice with H69 tumors are administered panobinostat. Three days after the initiation of panobinostat， and again 1 wk later， etoposide (40 mg/kg) is administered i.p.[1-5] |
| 数据来源文献 | [1]. Scuto A， et al. The novel histone deacetylase inhibitor， LBH589， induces expression of DNA damage response genes and apoptosis in Ph- acute lymphoblastic leukemia cells. Blood. 2008 May 15;111(10):5093-100. [2]. Crisanti MC， et al. The HDAC inhibitor panobinostat (LBH589) inhibits mesothelioma and lung cancer cells in vitro and in vivo with particular efficacy for small cell lung cancer. Mol Cancer Ther. 2009 Aug;8(8):2221-31. [3]. Ocio EM， et al. In vitro and in vivo rationale for the triple combination of panobinostat (LBH589) and dexamethasone with either bortezomib or lenalidomide in multiple myeloma. Haematologica. 2010 May;95(5):794-803. [4]. Banerjee NS， et al. Vorinostat， a?pan-HDAC?inhibitor，?abrogates?productive?HPV-18?DNA?amplification. Proc Natl Acad Sci U S A.?2018 Nov 20;115(47):E11138-E11147. [5]. Barton K， et al. Broad activation of latent HIV-1 in vivo. Nat Commun. 2016;7:12731. Published 2016 Sep 8. |
| 单位 | 瓶 |
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IP12901 帕比司他(10mM in DMSO,无菌） 抑制剂/拮抗剂/激动剂 索莱宝
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