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- 文献和实验
- 技术资料
- 保存条件：
Store at -20℃，6 months.
- 保质期：
Store at -20℃，6 months.
- 英文名：
beta-Lapachone(10mM in DMSO，Sterile)
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- CAS号：
4707-32-8
- 规格：
1ml
| CAS | 4707-32-8 |
| 中文名称 | β-兰帕科恩(10mM in DMSO，无菌） |
| 英文名称 | beta-Lapachone(10mM in DMSO，Sterile) |
| 分子式 | C15H14O3 |
| 分子量 | 242.27 |
| 规格 | 1ml |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Store at -20℃，6 months. |
| 运输条件 | 冷冻运输 |
| SMILES | CC1(CCC2=C(O1)C3=CC=CC=C3C(=O)C2=O)C |
| InChIKey | QZPQTZZNNJUOLS-UHFFFAOYSA-N |
| InChI | InChI=1S/C15H14O3/c1-15(2)8-7-11-13(17)12(16)9-5-3-4-6-10(9)14(11)18-15/h3-6H，7-8H2，1-2H3 |
| PubChem CID | 3885 |
| 靶点 | Topoisomerase |
| 通路 | DNA Damage/DNA Repair |
| 背景说明 | β-Lapachone 是一种萘醌类化合物，是拓扑异构酶 I (topoisomerase I) 抑制剂。 |
| 生物活性 | β-Lapachone (ARQ-501;NSC-26326) is a naturally occurring O-naphthoquinone， acts as a topoisomerase I inhibitor， and induces apoptosis by inhibiting cell cycle progression.[1-3] |
| In Vitro | β-Lapachone is a topoisomerase I inhibitor. β-Lapachone (25 μM) inhibits camptothecin-induced DNA cleavage[1]. β-Lapachone (10-40 μM) significantly reduces the colony-forming ability of CHO cells， and is cytotoxic in S phase. β-Lapachone at above 10 μM， causes a heavy DNA-strand breaks in CHO cells[2]. β-Lapachone (10 μM) suppresses JCPyV replication in IMR-32 cells. β-Lapachone (1.0?μM) potently affects JCPyV propagation in JCI cells. β-Lapachone (0.01-0.1 μM) inhibits VP1 production in JCI cells[4]. |
| In Vivo | β-Lapachone (0.066%) ameliorates cisplatin-induced renal injury and when in combination with cisplatin， the affect is more significant in mice. β-Lapachone increases Mre11-Rad50-Nbs1 (MRN) complex expression in mice[3]. |
| 细胞实验 | Cytotoxicity is measured by an MTT assay. IMR-32 and JCI cells are plated in 96-well microtiter plates at a concentration of 5.0?×?104 (topotecan) or 2.5?×?104 (β-lapachone) cells/well/100?μL medium 24 hr prior to addition of various concentrations of topotecan or β-lapachone. The cells are then incubated for 72?hr at 37°C in a CO2 incubator. Cell proliferation is assessed using a Cell Proliferation Kit I. Experiments are performed using four independent cultures[4]. |
| 动物实验 | Male Balb/c mice are provided a commercial pellet diet and water ad libitum. After 1 week of acclimation， the mice are randomly allocated to one of the following groups (5 per group): control， β-lapachone， cisplatin (18 mg/kg， ip)， and β-lapachone + cisplatin (18 mg/kg， ip). The β-lapachone groups are fed a diet containing the drug (0.066) for 2 weeks prior to cisplatin injection. All mice are sacrificed under carbon dioxide anesthesia 3 days after cisplatin injection. The blood samples are subjected to serum BUN and CRE analyses. Half of the kidney is quickly removed for histopathological and immunohistochemical (IHC) studies. The other half is stored at ?70°C until western blot assay[3]. |
| 激酶实验 | DNA topoisomerase I is incubated in the presence or absence of drugs (including β-Lapachone)， in 20 μL of relaxation buffer (50 mM Tris (pH 7.5). 50 mM KCl， 10 mM MgCl2， 0.5 mM dithiothreitol， 0.5 mM EDTA， 30 μg/mL bovine serum albumin) for 30 min at 37°C. Reactions are stopped by adding 1% SDS and proteinase K (50 μg/mL). After an additional 1-h incubation at 37°C， the products are separated by electrophoresis in 1% agarose gel in TAE buffer (0.04 M tris acetate， 0.001 M EDTA). The gel is stained with ethidium bromide after electrophoresis. The photographic negative is scanned with an NIH image analysis system[1]. |
| 数据来源文献 | [1]. Li CJ， et al. beta-Lapachone， a novel DNA topoisomerase I inhibitor with a mode of action different from camptothecin. J Biol Chem. 1993 Oct 25;268(30):22463-8. [2]. Vanni A， et al. DNA damage and cytotoxicity induced by beta-lapachone: relation to poly(ADP-ribose) polymerase inhibition. Mutat Res. 1998 Jun 5;401(1-2):55-63. [3]. Kim TW， et al. β-Lapachone enhances Mre11-Rad50-Nbs1 complex expression in cisplatin-induced nephrotoxicity. Pharmacol Rep. 2016 Feb;68(1):27-31. |
| 单位 | 瓶 |
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文献和实验商品化试剂和培养基均经过严格的质量控制以保证其无菌，但它们在操作过程中可能被污染。请遵循以下指导原则进行无菌操作，避免污染。请始终使用适当的灭菌方法（如高压灭菌器、除菌过滤器）对实验室中配制的任何试剂、培养基或溶液进行灭菌。 无菌操作 请始终用 70% 乙醇擦拭双手和工作区。 将容器、培养瓶、培养板和培养皿放入细胞培养通风橱之前，先用 70% 乙醇擦拭其外部。 不要直接从试剂瓶或培养瓶中倾倒培养基和试剂。 使用无菌玻璃或一次性塑料移液管和移液器操作液体时，每只移液管只能使用一次，以避免
培养基经高压灭菌后，用经过灭菌的工具（如接种针和吸管等）在无菌条件下接种含菌材料（如样品、菌苔或菌悬液等）于培养基上，这个过程叫做无菌接种操作。在实验室检验中的各种接种必须是无菌操作。 实验台面不论是什么材料，一律要求光滑、水平。光滑是便于用消毒剂擦洗；水平是倒琼脂培养基时利于培养皿内平板的厚度保持一致。在实验台上方，空气流动应缓慢，杂菌应尽量减少，其周围杂菌也应越少越好。为此，必须清扫室内，关闭实验室的门窗，并用消毒剂进行空气消毒处理，尽可能地减少杂菌的数量
多聚-β-羟丁酸poly-β-hydroxybutyricacid
几种芽孢杆菌属（Bacillus），假单胞菌属（Pseudomonas）、螺菌属（Spirillum）、固氮菌属（Azotobacter）以及其他许多种细菌在菌体内制造的贮藏物质。 在光学显微镜下可以因折光或呈颗粒状而被观察到，并可用苏丹黑染色。在细菌中作为代替油脂贮藏物，而合成此化合物。一般在培养基中氮源缺乏而碳源多的条件下合成，如果供给氮化合物，水解成单体的β-羟丁酸后，经过乙酰乙酸、乙酰CoA，被利用来合成生物体内各种成分
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