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文献和实验A Yeast-Based Recombination Assay for Homing Endonuclease Activity
Homing endonucleases (HEs) are natural enzymes that cleave long DNA target with a high specificity and trigger homologous recombination at the exact site of the break. Such mechanisms can thus be used for all the applications covered today
Identification and Analysis of Genomic Homing Endonuclease Target Sites
Homing endonucleases (HEs) are highly site-specific enzymes that enable genome engineering by introducing DNA double-strand breaks (DSB) in genomic target sites. DSB repair from an HE-induced DSB can promote target site gene deletion
Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile
Evaluating the binding specificity and identifying the most preferred target sequence for a homing endonuclease often represents a key step during its characterization, engineering, and application for genome engineering. This chapter
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