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- 供应商:
依诺赞(江苏)生物科技有限公司
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200rxns
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文献和实验A multiplexed glyco-bead array method for determining the sugar-binding specificities of plant lectins was described by a bead-based flow cytometric assay. Glycopeptides with N - and O -glycans were immobilized on multiplexed beads
Farnham Lab Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues
. If necessary, add more PBS (plus protease inhibitors) to tissue for a homogeneous suspension. Check cell suspension by microscope. Centrifuge cells at 1000 rpm at 4 o C. Measure cell pellet volume. Chromatin Preparation
Protein Expression and Purification of RPA70-AB (181-422)
mM phosphate,0.15 M NH4Cl,2% glycerol pH 7.4,O/N in the cold room.Before adding protease save some undigested sample for SDS-PAGE.15.Check the sample by SDS-PAGE for the completion of the digestion.16.Pass the sample over Ni-NTA column equilibrated
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