Measurement of the Sugar-Binding Specificity of Lectins Using Multiplexed Bead-Based Suspension Arrays

A multiplexed glyco-bead array method for determining the sugar-binding specificities of plant lectins was described by a bead-based flow cytometric assay. Glycopeptides with N - and O -glycans were immobilized on multiplexed beads, and the specificities of several kinds of sugar chains were measured in a single reaction. This strategy is easy, rapid, reproducible, and suitable for small samples, and allows the reliable elucidation of sugar-binding properties of lectins under identical conditions.