4-(4'-Carboxyphenyl)piperidine

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region

p27 ELISA

Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells

Tris缓冲液（TBS和THB）的配制

一、TBS的配制 0.05 M TBS ( pH7.4 ) 的配制： Tris ( 三羟甲基胺基甲烷 ) 12.1g NaCl 17.5g 加蒸馏水 1500ml 磁性搅拌下滴加浓HCl至pH为7.4，再加蒸馏水至2000ml，即可。 如需含1% Triton X-100，则在滴加HCl前先加入20