NED 染料 qPCR 校准溶液 *10,000X*

产品基本信息

产品名称：NED 染料 qPCR 校准溶液 *10,000X*

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：NA

溶剂：水

激发波长(nm)：545

发射波长(nm)：567

 

产品介绍

实时热循环仪需要校准才能生成荧光信号的光谱图。由于仪器的荧光检测灵敏度可能不同，校准任何染料（如 NED）所需的浓度将取决于所使用的设备。 AAT Bioquest NED 染料 qPCR 校准溶液以 10,000X 浓缩液形式提供，与多种 qPCR 仪器兼容，包括 ABI7500 Fast、QuantStudio™ 和 ViiA™ 7 系统。校准和验证应每六个月或根据需要运行一次。，为您提供优质的NED 染料 qPCR 校准溶液 *10,000X*。 

点击查看光谱

 

实验方案

此通用协议适用于 ABI7500 FAST qPCR 仪器。

准备 NED 校准板：

1.将 1 μL 的 10,000X NED 染料 qPCR 校准溶液与 10 mL 的无核酸酶水或 qPCR 缓冲液混合，制备 NED 染料 qPCR 校准工作溶液。请注意，染料浓度必须根据仪器的校准要求进行优化。

2.在反应板的所有孔中加入 20 μL 的 NED Dye qPCR 校准工作溶液。

3.用光学胶盖密封板

4.以 < 1500 rpm 的速度将板离心 2 分钟

5.检查板的每个孔中的液体是否位于孔的底部。如果没有，请再次以稍高的 rpm 和更长的时间将板离心

6.有关完整的校准说明，请参考您仪器的维护指南

 

参考文献

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Journal: Talanta (2021): 122187

Fluorescent Probe for Ag+ Detection Using SYBR GREEN I and C-C Mismatch.
Authors: Zhou, Xiaohong and Memon, Abdul Ghaffar and Sun, Weiming and Fang, Fang and Guo, Jinsong
Journal: Biosensors (2020)

Comparison of Retrograde, Primary and Secondary Bonding Materials with Tooth Substance.
Authors: Khan, Tabinda Nawaz and Abidi, Syed Yawar Ali
Journal: Journal of the College of Physicians and Surgeons--Pakistan : JCPSP (2018): 9-12

Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82

Micromechanical Intervention in Sandwich Restoration.
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4-Aminothiophenol functionalized gold nanoparticle-based colorimetric sensor for the determination of nitramine energetic materials.
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