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- 详细信息
- 技术资料
- 英文名：
VIC Dye qPCR Calibration Solution *10,000X*
- 库存：
50
- 供应商：
广州市左克生物科技发展有限公司
- 规格：
100ul
产品参数
| Ex (nm) | 526 | Em (nm) | 543 |
| 分子量 | N/A | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品基本信息
产品名称：VIC 染料 qPCR 校准溶液 *10,000X*
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：NA
溶剂：水
激发波长(nm)：526
发射波长(nm)：543
产品介绍
实时热循环仪需要校准才能生成荧光信号的光谱图。由于仪器的荧光检测灵敏度可能不同，校准任何染料（如 VIC）所需的浓度将取决于所使用的设备。 AAT Bioquest VIC 染料 qPCR 校准溶液以 10,000X 浓缩液形式提供，可与多种 qPCR 仪器兼容，包括 ABI7500 Fast、QuantStudio™ 和 ViiA™ 7 系统。校准和验证应每六个月或根据需要运行一次。，为您提供优质的VIC 染料 qPCR 校准溶液 *10,000X*。
点击查看光谱
实验方案
此通用协议适用于 ABI7500 FAST qPCR 仪器。
准备 VIC 校准板：
1.将 1 μL 的 10,000X VIC 染料 qPCR 校准溶液与 10 mL 的无核酸酶水或 qPCR 缓冲液混合，制备 VIC 染料 qPCR 校准工作溶液。请注意，染料浓度必须根据仪器的校准要求进行优化。
2.在反应板的所有孔中加入 20 μL 的 VIC Dye qPCR 校准工作溶液。
3.用光学胶盖密封板
4.以 < 1500 rpm 的速度将板离心 2 分钟
5.检查板的每个孔中的液体是否位于孔的底部。如果没有，请再次以稍高的 rpm 和更长的时间将板离心
6.有关完整的校准说明，请参考您仪器的维护指南
参考文献
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Influence of Sequential Liquid Ammonia and Caustic Mercerization Pre-Treatment on Dyeing Performance of Knit Cotton Fabric.
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Journal: Materials (Basel, Switzerland) (2022)
Authors: Shelke, Harshad D and Machale, Archana R and Survase, Avinash A and Pathan, Habib M and Lokhande, Chandrakant D and Lokhande, Abhishek C and Shaikh, Shoyebmohamad F and Rana, Abu Ul Hassan S and Palaniswami, Marimuthu
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Antimicrobial activity of sophorolipids against Eimeria maxima and Clostridium perfringens, and their effect on growth performance and gut health in necrotic enteritis.
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VIC 染料 qPCR 校准溶液 *10,000X*
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