生物素酪胺 CAS 41994-02-9

产品基本信息

产品名称：生物素酪胺

CAS：41994-02-9

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：363.48

溶剂：DMSO

 

产品介绍

对于许多免疫组织化学（IHC）的应用，传统的酶扩增程序足以实现充分的抗原检测。然而，有几个因素限制了这些程序的敏感性和实用性。酪胺信号放大技术（TSA）已被证明是一种特别通用和强大的酶放大技术，提高了检测灵敏度。TSA是基于HRP在低浓度过氧化氢存在下将标记的酪胺底物转化为氧化的、高活性的自由基的能力，该自由基可在HRP处或其附近共价结合酪氨酸残基。这一过程被称为催化报告沉积（CARD），通过每个过氧化物酶标签上的多个酪胺底物的转换所产生的信号放大转化为对低丰度目标的超灵敏检测和使用少量抗体和杂交探针。在免疫组化应用中，TSA法的敏感性增强使原代抗体稀释度增加，以减少非特异性背景信号，并能克服由于固定程序不理想或靶蛋白表达水平低而导致的弱免疫标记。生物素酪胺被认为能在所有TSA试剂中实现大限度的IHC检测。生物素酪胺已用于免疫组织化学、ELISA、Western blot和原位杂交应用。，为您提供优质的生物素酪胺。

 

参考文献

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