相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Biotin-PEG4-tyramide
- 库存:
50
- 供应商:
广州市左克生物科技发展有限公司
- 规格:
200Slides
| Ex (nm) | - | Em (nm) | - |
| 分子量 | 610.77 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品基本信息
产品名称:生物素-PEG4-酪胺
储存条件:-15℃避光防潮
保质期:12个月
注:200slides:一管试剂足够200个玻片使用;注2:200slides大约为100ug。
产品物理化学光谱特性
分子量:610.77
溶剂:DMSO
产品介绍
生物素-PEG4-酪胺是一种通过催化报告分子沉积(CARD)用于酪胺信号放大(TSA)的试剂。在CARD中,报告酶,例如与二级抗体结合的辣根过氧化物酶(HRP),与目标结合并催化生物素酪胺共价沉积到样品上。然后用检测(如链霉亲和素HRP)样品,允许通过显色或荧光方法进行检测。生物素-PEG4-酪胺已被用于免疫组织化学、ELISA、Western blot和原位杂交应用。在大多数生物检测中,它往往比生物素酪胺具有更好的信号/背景。,为您提供优质的生物素-PEG4-酪胺。
参考文献
High specificity and tight spatial restriction of self-biotinylation by DNA and RNA G-Quadruplexes complexed in vitro and in vivo with Heme.
Authors: Lat, Prince Kumar and Liu, Kun and Kumar, Dev N and Wong, Kenneth K L and Verheyen, Esther M and Sen, Dipankar
Journal: Nucleic acids research (2020)
APEX Peroxidase-Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics.
Authors: Kalocsay, Marian
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 41-55
Biotinylation and isolation of an RNA G-quadruplex based on its peroxidase-mimicking activity.
Authors: Li, Wei and Zeng, Weiwu and Chen, Yi and Wang, Fang and Wu, Fan and Weng, Xiaocheng and Zhou, Xiang
Journal: The Analyst (2019): 4472-4476
Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2,6 sialic acid-dependent and -independent interactions.
Authors: Alborzian Deh Sheikh, Amin and Akatsu, Chizuru and Imamura, Akihiro and Abdu-Allah, Hajjaj H M and Takematsu, Hiromu and Ando, Hiromune and Ishida, Hideharu and Tsubata, Takeshi
Journal: Biochemical and biophysical research communications (2018): 854-859
Identification of Siglec Ligands Using a Proximity Labeling Method.
Authors: Chang, Lanyi and Chen, Yi-Ju and Fan, Chan-Yo and Tang, Chin-Ju and Chen, Yi-Hsiu and Low, Penk-Yeir and Ventura, Albert and Lin, Chun-Cheng and Chen, Yu-Ju and Angata, Takashi
Journal: Journal of proteome research (2017): 3929-3941
Tyramide Signal Amplification Permits Immunohistochemical Analyses of Androgen Receptors in the Rat Prefrontal Cortex.
Authors: Low, Katelyn L and Ma, Chunqi and Soma, Kiran K
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2017): 295-308
Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis.
Authors: Royston, Daniel J and Clasper, Steven and Jackson, David G
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 35-48
Development of a highly sensitive immunohistochemical method to detect neurochemical molecules in formalin-fixed and paraffin-embedded tissues from autopsied human brains.
Authors: Goto, Satoshi and Morigaki, Ryoma and Okita, Shinya and Nagahiro, Shinji and Kaji, Ryuji
Journal: Frontiers in neuroanatomy (2015): 22
Development of a sensitive ELISA for quantification of three- and four-repeat tau isoforms in tauopathies.
Authors: Luk, Connie and Giovannoni, Gavin and Williams, David R and Lees, Andrew J and de Silva, Rohan
Journal: Journal of neuroscience methods (2009): 34-42
Levels of pregnancy-associated plasma protein A in patients with coronary artery disease.
Authors: Liu, Zhi-Yuan and Zhang, Jin-Ying and Sun, Tong-Wen and Zhang, Yan-Jun and Zhang, Li and Wang, Le-Xin
Journal: Clinical and investigative medicine. Medecine clinique et experimentale (2008): E85-9
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验信号减弱。 检测膜蛋白一般不建议使用甲醇或丙酮固定,有机溶剂能够破坏膜结构。 4. 透化方法不当 检查靶标蛋白表达位置,胞内蛋白需要透化处理。 膜蛋白需要考虑所检测靶表位位于膜内还是膜外,膜外则不需要透化。 5. 二抗选择不当 二抗选择建议如下: 6. 信号放大不够 如果检测靶点是低丰度蛋白.信号弱可能是由于信号放大不足导致的,可以使用Invitrogen SuperBoost 酪胺信号放大技术,进一步增强信号,实现低丰度,难以检测蛋白的检测
。四、二次酶放大时间分辨荧光分析 近年又提出二次酶放大时间分辨荧光分析系统[3,6],用于核酸杂交分析。其原理如下:先用抗体包被微滴定孔,再经特异抗原标记的靶DNA与该抗体反应,将靶DNA连接到固相抗体上。然后用生物素化探针与靶DNA杂交,再通过生物素与链霉亲和素的反应,将链霉亲和素标记的辣根过氧化物酶(HRP)连接到杂化物上。当加入含有H2O2和生物素化酪胺(B-T)的HRP的底物溶液,因有H2O2存在,HRP在催化酪胺氧化,产生游离基团的同时,也催化事先固定在微滴定孔表面的蛋白质(如白蛋白
I2 ),再与多肽激素、蛋白质分子中的酪氨酸残基发生碘化作用。所以不管采用哪一种放射性碘标记法,标记的化合物内部必须有碘原子可结合的基团,即结构上要含有酪胺基或组织胺残基。凡蛋白质、肽类等抗原,在结构上含有上述基团的可直接用放射性碘进行标记。如不含上述基团的,放射性碘无法标记,必须在这些化合物的结构上连接上述基团后才能进行碘标记。 因此影响蛋白质、多肽碘化效率的因素,主要决定于蛋白质、多肽分子中酪氨酸残基的数量及它们在分子结构中暴露的程度;此外,碘化物的用量、反应条件(pH、温度、反应
技术资料暂无技术资料 索取技术资料




![Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2',3'-dideoxyadenosine-5'-triphosphate] CAS 114748-69-5](https://img1.dxycdn.com/p/s14/2025/0711/840/2743138700649953491.jpg!wh200)


