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Cell Meter RX700固定化细胞活性染料

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  • ¥2202
  • AAT Bioquest
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  • 22532
  • 2025年07月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 英文名

      Cell Meter™ RX700 fixable viability dye

    • 库存

      50

    • 供应商

      广州市左克生物科技发展有限公司

    • 规格

      200Tests

    产品参数
    Ex (nm) 690 Em (nm) 713
    分子量 - 溶剂 DMSO
    存储条件 在零下15度以下保存, 避免光照
    产品概述

    产品基本信息

    产品名称:Cell Meter RX700固定化细胞活性染料

    储存条件:保存在冰箱-15℃干燥

    保质期:12个月

     

    产品介绍

    从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter RX700固定化细胞活性染料是一类细胞不可渗透的荧光活性染料,它们经过优化以匹配常见流式细胞仪的主要激发激光,例如350、405、488、633和647 nm。这些染料不渗入活细胞,但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter RX700固定化细胞活性染料发生反应,因此比具有完整膜的活细胞的染色更亮,因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时,需要考虑一些因素,例如,每种染料的滴定,以确保活细胞几乎没有染色。Cell Meter RX700固定化细胞活性染料经过优化,可以在633/647 nm的红色激光激发下以700 nm的波长发射。与其他商业上类似的活性染料相比,这种固定化细胞活性染料更加耐用、稳定。,为您提供 优质的Cell Meter RX700固定化细胞活性染料。 

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    参考文献

    CFSE dilution to study human T and NK cell proliferation in vitro.
    Authors: Terrén, Iñigo and Orrantia, Ane and Vitallé, Joana and Zenarruzabeitia, Olatz and Borrego, Francisco
    Journal: Methods in enzymology (2020): 239-255

    PGC-1α Regulates Cell Proliferation and Invasion via AKT/GSK-3β/β-catenin Pathway in Human Colorectal Cancer SW620 and SW480 Cells.
    Authors: Yun, Seong-Hoon and Park, Joo-In
    Journal: Anticancer research (2020): 653-664

    The miR-3940-5p inhibits cell proliferation of gingival mesenchymal stem cells.
    Authors: Han, Xiao and Yang, Haoqing and Cao, Yangyang and Ge, Lihua and Han, Nannan and Zhang, Chen and Fan, Zhipeng and Yao, Rui
    Journal: Oral diseases (2019): 1363-1373

    Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing.
    Authors: Nagarkatti, Mitzi and Rieder, Sadiye Amcaoglu and Nagarkatti, Prakash S
    Journal: Methods in molecular biology (Clifton, N.J.) (2018): 209-230

    Toll-Like Receptor 4 (TLR4)/Cyclooxygenase-2 (COX-2) Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion by NF-κB Activation.
    Authors: Wang, Wei and Wang, Jiye
    Journal: Medical science monitor : international medical journal of experimental and clinical research (2018): 5588-5597

    Gag-Specific CD4 T Cell Proliferation, Plasmacytoid Dendritic Cells, and Ethnicity in Perinatally HIV-1-Infected Youths: The ANRS-EP38-IMMIP Study.
    Authors: Scott-Algara, Daniel and Warszawski, Josiane and Chenadec, Jérôme Le and Didier, Céline and Montange, Thomas and Viard, Jean-Paul and Dollfus, Catherine and Avettand-Fenoel, Véronique and Rouzioux, Christine and Blanche, Stéphane and Buseyne, Florence
    Journal: AIDS research and human retroviruses (2017): 21-28

    Quantitative analysis of cell proliferation by a dye dilution assay: Application to cell lines and cocultures.
    Authors: Chung, Soobin and Kim, Seol-Hee and Seo, Yuri and Kim, Sook-Kyung and Lee, Ji Youn
    Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2017): 704-712

    [Myeloid-derived suppressor cells promoted autologous B cell proliferation in rheumatoid arthritis].
    Authors: Chen, W and Hu, F L and Liu, H J and Xu, L L and Li, Y N and Li, Z G
    Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2017): 819-823

    A comparative study of colorimetric cell proliferation assays in immune cells.
    Authors: Koyanagi, Madoka and Kawakabe, So and Arimura, Yutaka
    Journal: Cytotechnology (2016): 1489-98

    Hypoxia inhibits mesenchymal stem cell proliferation through HIF1α-dependent regulation of P27.
    Authors: Kumar, Sanjay and Vaidya, Meenal
    Journal: Molecular and cellular biochemistry (2016): 29-38

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    图标文献和实验
    相关实验
    • Transformation of E. coli by Electroporation

      autoclaved bottles!). 4. Wash the cell pellet by resuspending in 250 ml of sterile ice-cold WB (10% redistilled glycerol, 90% distilled water, v/v). Centrifuge the cell suspension at 5,000 RPM for 15 min and carefully pour off the supernate as soon

    • 基本实验技术

      . If the solution needs to be at a specific pH, check the pH meter with fresh buffer solutions and follow instructions for using a pH meter. Autoclave, if possible, at 121EC for 20 min. Some solutions cannot be autoclaved, for example, SDS

    • Spectrophotometry

      . Thus, there is a relationship between the voltage and the amount of dye in the sample. Given the geometry of a spectrophotometer, what is actually measured at the photocell is the amount of light energy which arrives at the cell. The voltage meter is reading

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