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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Tetrasaccharide
- 保质期:
365
- 英文名:
6`-suSiaLex-C3-biot
- 库存:
5
- 供应商:
艾美捷
- CAS号:
无
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文献和实验tianjiaoya 小弟最近要构建一个质粒,需要用到Gal4转录因子,有哪位大侠知道Gal4的UAS在那个质粒上?最好是容易买到的质粒啊,我查了好多质粒,都很难买到。或者能把Gal4的UAS的序列告诉我,这个上游激活序列应该不大,我可以去直接人工合成,然后参照别人那些构建成功的质粒的样子,把UAS插入到我的质粒中去,谢谢各位大侠了!!! song333 GAL4/UAS system The GAL
Beta-gal Assay Adapted from Current Protocols in Molecular Biology 1. Grow 5ml YEPD cultures to mid-log phase. 2. Centrifuge and resuspend cells in 5ml Z-buffer, then place on ice. 3. Measure OD600. 4. Use straight
, transfect the DNA into mammalian cells, and then fix and stain a tissue with X-gal. The cells that turn blue must have the beta galactosidase enzyme, the product of the lac Z gene, which could only have been expressed from the donated promoter. Ergo
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