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- PVT1406
- 2026年03月16日
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- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pRevTRE
- 库存：
10
- 供应商：
LSMBIO
- 规格：
质粒干粉
pRevTRE
Search name
pRevTRE,Plasmid pRevTRE,pRevTRE vector
pRevTRE Information
Promoter: CMV
Replicon: pUC ori
Plasmid classification: mammalian cells, tetracycline system vector.
Plasmid size: 6487bp
Prokaryotic resistance: Amp
Screening markers: Hyg
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Inducement: tetracycline induction
5'sequencing primers: primers designed according to sequence
3'sequencing primers: primers designed according to sequence
pRevTRE閳?/strong> Description
pRevTRE is a retroviral Tet response vector that expresses a gene of interest from the Tetresponse element (TRE). This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV) as described (1). The TRE contains seven direct repeats of the tetO operator sequence, upstream of a minimal CMV promoter, which can be bound by the tTA and rtTA transactivators. The 5' viral LTR controls expression of the transcript that contains 唯+ (the extended viral packaging signal) and the hygromycin resistance (Hygr) gene for antibiotic selection in mammalian cells. The TRE is derived from vectors described previously (2, 3). pRevTRE also includes the E. coli Ampr gene for antibiotic selection in bacteria.
pRevTRE閳?Sequence
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文献和实验This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
质粒 plasmid 在细胞内整个世代稳定地传递给后代，但并不在染色体上而进行自主增殖的一类遗传因子的总称。该类因子的存在与否，对细胞的生存无关紧要。这个名词主要指细菌细胞内的因子，它们包括可在细胞间发生传递的F因子、 R因子、大肠杆菌素因子等。此外，还有各种本身不能传递的因子。在具有质粒细胞的后代中，有时会出现低频率的失去质粒的细菌。在质粒中，对那些能变成插入奇主染色体状态的特殊质粒，特称为附加体（episome）。
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