- ￥5099
- 赛默飞（Thermo Fisher）
- 136200
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 规格：
200 UG
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
per 200 ml of original cell growth is approximately 150 ug.
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells
is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
like to increase the concentration of cDNA to 200 ng/ul but am wondering if this can cause any problems. I use SYBR green. Also, my water blank with reference gene(18S) has a CT value of 32.0. I have run this with DEPC water, and two different bottles
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