- ￥4225
- 赛默飞（Thermo Fisher）
- 82380
- 2025年07月16日
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文献和实验. 4. When finished, immerse membrane in Blocking buffer and block overnight. H. Antibodies and detection 1. Incubate with primary antibody diluted in Blocking buffer for 2 hours at room temp. 2. Wash 3 x 10 min with PBST. 1 x10min secondary washing
. Add 50ul of each of the above dilutions to 25ul of 3x strength titre of antibody (i.e. a solution 3 x the titre giving 50% inhibition on direct ELISA), in sealed tubes. 3. Incubate overnight at room temperature. 4. Set up competition
of each of the above dilutions to 25ul of 3x strength titre of antibody (i.e. a solution 3 x the titre giving 50% inhibition on direct ELISA), in sealed tubes. 3. Incubate overnight at room temperature. 4. Set up competition between unknown concentration of competitive
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