Ultra-LEAF™ Purified anti-VEGF

Ultra-LEAF™ Purified anti-VEGF-121 Antibody
A15136B
Catalog# / Size 692203 / 100 µg
692204 / 1 mg
Clone
Regulatory Status RUO
Other Names VEGFA, MVCD1, VEGF, Vascular permeability factor (VPF)
Isotype Mouse IgG2b, κ
Description VEGF was initially identified in conditioned medium from bovine pituitary follicular cells. VEGFA belongs to the VEGF family, which has the following members: VEGF-A, VEGF-B, VEGF-C,
VEGF-D, and PlGF (placental growth factor). In addition, viral VEGF homologs (collectively
called VEGF-E) and snake venom VEGFs such as T.f. (Trimeresurus flavoviridis) and svVEGF
(called VEGF-F) have been described. VEGFA is alternatively spliced to generate variants with
different number of amino acids such as VEGF-121, VEGF-145, VEGF-165, and VEGF-189.
While VEGF-121 is freely diffusible and does not bind to heparin sulphate, VEGF-165 and
VEGF-189 bind to heparin sulfate, resulting in retention on the cell surface or in the
extracellular matrix. VEGF-A is highly expressed in solid tumors generated in breast, lung,
renal, colorectal and liver tissues. VEGF has strong vascular permeability activity and
significantly contributes to the formation of ascites tumors. VEGF can act as a direct
proinflammatory mediator during the pathogenesis of rheumatoid arthritis (RA) and protect
rheumatoid synoviocytes from apoptosis, which contributes to synovial hyperplasia. VEGF is
expressed in synovial macrophages and synovial fibroblasts in RA patients. Also, VEGF is
associated with age-related macular degeneration (AMD). AMD is due to neovascularization
that originates from endothelial cells in the choroid that grow into neurosensory retina as
choroidal neovascularization (CNV).
Product Details
Verified Reactivity Human
Antibody Type Monoclonal
Host Species Mouse
Immunogen Recombinant human VEGF-121.
Formulation Phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3
mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and
expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™
solution contains no preservative; handle under aseptic conditions