Purified (azide-free) anti-Neu

Purified anti-Neurofilament H (NF-H), Nonphosphorylated Antibody (Previously Covanc
catalog# SMI-32P)
SMI 32
Catalog# / Size 
801701 / 100 µL
Clone
Regulatory Status RUO
Other Names Neurofilament heavy polypeptide, NF-H, 200 kD neurofilament protein, neurofilament triplet H protein
Previously Covance Catalog# SMI-32P
Isotype Mouse IgG1
Description Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are
a major component of the neuronal cytoskeleton, and function primarily to provide structural support for
the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to
these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE.
The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160
kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of
these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The
level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals
travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic
lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for
differentiating neurons (positive for NF) from the glia (negative for NF).