- ￥3007
- BioLegend
- 105814
- 美国
- 2025年07月10日
- 免疫荧光，流式
- 大鼠
- 小鼠
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- 详细信息
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- 技术资料
- 抗体名：
PE/Cyanine7 anti-mouse CD117 (c-kit)
- 抗体英文名：
PE/Cyanine7 anti-mouse CD117 (c-kit)
- 浓度：
0.2 mg/ml
- 应用范围：
免疫荧光，流式
- 宿主：
大鼠
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
大鼠
- 保质期：
2-3年左右
- 适应物种：
小鼠
- 标记物：
PE/Cyanine7
- 克隆性：
单克隆
- 保存条件：
2-8度
- 形态：
液体
- 规格：
100 μg
PE/Cyanine7 anti-mouse CD117 (c-Kit) Antibody
2B8
Catalog# / Size 105813 / 25 µg
105814 / 100 µg
Clone
Regulatory Status RUO
Other Names c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype Rat IgG2b, κ
Description CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell
factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit
ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is
expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells,
progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast
cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for
the development of hematopoietic stem cells.
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Mouse bone marrow mast cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under
optimal conditions.
Concentration 0.2 mg/mL
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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文献和实验更多产品详细信息欢迎来电咨询~周祥15900853496（微信同号)，汪海洋15901695283(微信同号)
A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
中。5. 用无菌 PBS 将上述锥形试管填满（至 50 mL)。介绍样品制备。6. 洗涤。在室温、低速制动条件下，以 300 X g 离心分离试管 10 分钟。7. 裂解红细胞（BD 产品目录号：555899，10 x 溶液。在蒸馏水中使用 1 x 溶液。室温孵育 15 分钟。）8. 将 1x106 PBMC 重悬于 20 μL 染色缓冲液中（PBS 含有 5% 正常人血清）。9. 加入抗体：CD117-BV421 (c-kit)、CD7-BV510、CD57- BV605、CD8-BV650
C-kit Expression Identifies Cardiac Precursor Cells in Neonatal Mice
receptor c-kit. Utilizing a bacterial artificial chromosome (BAC) transgenic mouse model in which EGFP expression is placed under control of the c-kit promoter (c-kitBAC -EGFP), work from our laboratory indicates that c-kit expression identifies
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